Generation of Protective T Cell-Independent Antiviral Antibody Responses in SCID Mice Reconstituted with Follicular or Marginal Zone B Cells
Generation of Protective T Cell-Independent Antiviral Antibody Responses in SCID Mice Reconstituted with Follicular or Marginal Zone B Cells
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DOI：
10.4049/jimmunol.0900068
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发表时间：
2009-07-01
影响因子：
4.4
通讯作者：
Szomolanyi-Tsuda, Eva
中科院分区：
文献类型：
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作者：
Guay, Heath M.;Mishra, Rabinarayan;Szomolanyi-Tsuda, Eva
成年小鼠骨髓中产生的B细胞作为过渡性B细胞进入外周，随后分化成两种表型和功能不同的亚群之一，边缘区（MZ）或滤泡（Fo）B细胞。然而，最近的报道表明，响应于环境因素，如淋巴细胞减少，成熟的Fo B细胞可以改变以显示MZ B细胞的表型标志物特征。以前，我们发现转移到SCID小鼠的脾B细胞对多瘤病毒（PyV）感染有反应，具有T细胞依赖性（TI）IgM和IgG分泌，减少病毒载量并保护小鼠免受感染的致死作用。然而，MZ和Fo B细胞亚群对这种抗病毒TI-2应答的贡献尚未得到解决。在这项研究中，我们表明，分选纯化的MZ和Fo B细胞转移到SCID小鼠时，对PyV感染产生保护性TI Ab反应。此外，在PyV感染的SCID小鼠的脾脏中转移的Fo B细胞改变表型，其中许多显示MZ B细胞特征。这些发现证明了病毒感染宿主中B细胞亚群的可塑性，并首次表明完全来源于Fo B细胞的B细胞可有效地在抗病毒TI-2应答中发挥作用。免疫学杂志，2009，183：518-523.
B cells generated in the bone marrow of adult mice enter the periphery as transitional B cells and subsequently differentiate into one of two phenotypically and functionally distinct subsets, marginal zone (MZ) or follicular (Fo) B cells. Recent reports indicate, however, that in response to environmental cues, such as lymphopenia, mature Fo B cells can change to display phenotypic markers characteristic of MZ B cells. Previously, we found that splenic B cells transferred to SCID mice responded to polyoma virus (PyV) infection with T cell-in dependent (TI) IgM and IgG secretion, reducing the viral load and protecting mice from the lethal effect of the infection. The contribution of MZ and Fo B cell subsets to this antiviral TI-2 response, however, has not been addressed. In this study, we show that both sort-purified MZ and Fo B cells generate protective TI Ab responses to PyV infection when transferred into SCID mice. Moreover, the transferred Fo B cells in the spleens of the PyV-infected SCID mice change phenotype, with many of them displaying MZ B cell characteristics. These findings demonstrate the plasticity of the B cell subsets in virus-infected hosts and show for the first time that B cells derived exclusively from Fo B cells can effectively function in antiviral TI-2 responses. The Journal of Immunology, 2009, 183: 518-523.