Measurements of the degradation products of radioiodinated proteins.
Measurements of the degradation products of radioiodinated proteins.
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放射性碘标记蛋白质降解产物的测量。
DOI：
10.1016/0003-2697(81)90556-x
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发表时间：
1981
影响因子：
2.9
通讯作者：
S. Hmiel
中科院分区：
文献类型：
--
作者：
G. Chisolm;C. Sila;S. Hmiel
本文提供的证据表明，如果在没有过量未标记的碘离子的情况下通过三氯乙酸沉淀来测定，则放射性碘标记的蛋白质样品中存在的放射性碘化物 (*I−) 的比例被严重低估。此外，广泛使用的从三氯乙酸沉淀物上清液中放射性碘标记的碘酪氨酸中分离*I-的方法，是通过氧化*I-并随后萃取到氯仿中进行的（24），这取决于所用三氯乙酸的浓度。浓度为 10% (w/v) 或更高时似乎可以获得重现性和准确性。本文中作为替代方法提出的 Goldberg 薄层色谱方法 (25) 的改进提供了一步分离 ∗ I−, ∗ I-Tyr 和放射性碘标记蛋白的简单性。该方法可用于尿液、血浆或组织匀浆的小样本，其中寻求对放射性碘标记的蛋白质的标记副产物进行定量。
This paper presents evidence that the fraction of radioactive iodide (∗I−) present in a sample of radioiodinated protein is grossly underestimated if determined by trichloroacetic acid precipitation in the absence of excess unlabeled iodide ion. In addition, the widely used separation of∗I−from radioactive iodine-labeled iodotyrosine in supernatants of trichloroacetic acid precipitates, which proceeds by oxidation of∗I−and subsequent extraction into chloroform (24), is dependent on the concentration of trichloroacetic acid used. Reproducibility and accuracy appear to be obtainable at concentrations of 10% (w/v) or higher. The modification of Goldberg's thin-layer chromatographic method (25) presented as an alternative method in the current paper offers the simplicity of separating∗I−,∗I-Tyr, and radioiodinated protein in a single step. The method can be used for small samples of urine, plasma, or tissue homogenates in which quantification of the labeled by-products from radioiodinated proteins is sought.
纯合子家族性高胆固醇血症中高密度脂蛋白的异常。
DOI：
10.1161/01.atv.4.5.472
发表时间：
1984
期刊：
Arteriosclerosis (Dallas, Tex.)
影响因子：
--
作者：
Goldberg,RB;Fless,GM;Baker,SG;Joffe,BI;Getz,GS;Scanu,AM;Seftel,HC
通讯作者：
Seftel,HC