The influence of nitrogen limitation on mcl-PHA synthesis by two newly isolated strains of Pseudomonas sp.
The influence of nitrogen limitation on mcl-PHA synthesis by two newly isolated strains of Pseudomonas sp.
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DOI：
10.1007/s10295-010-0698-5
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发表时间：
2010-05-01
影响因子：
3.4
通讯作者：
Przybylek, Grzegorz
中科院分区：
文献类型：
--
作者：
Ciesielski, Slawomir;Mozejko, Justyna;Przybylek, Grzegorz
对两种新分离的假单胞菌菌株中参与中链长度聚羟基脂肪酸酯 (mcl-PHA) 合成的关键酶的核苷酸组成进行了分析。测试了分离的菌株使用三种不同底物作为碳源合成聚羟基脂肪酸酯的能力：辛酸钠、油酸和葡萄糖酸钠。两种分析菌株都能积累 2.07% 至 21.40% 的 mcl-PHA，这取决于所使用的底物。通过在氮限制和非限制条件下培养细胞来分析 mcl-PHA 合成的潜在氮依赖性调节。分析的菌株表明，当使用油酸和葡萄糖酸钠作为碳源时，mcl-PHA 因氮饥饿而逐渐增加。转录分析表明，PHA 合酶基因编码的诱导与 mcl-PHA 含量的增加相关。两种分析的菌株都揭示了所研究基因表达的差异，显示出对所用碳源的依赖性。
The nucleotide composition of key enzymes involved in medium-chain-length polyhydroxyalkanoates (mcl-PHA) synthesis was analyzed in two newly isolated strains of Pseudomonas. The isolated strains were tested for their abilities to synthesize polyhydroxyalkanoates using three different substrates as a carbon source: sodium octanoate, oleic acid, and sodium gluconate. Both analyzed strains were able to accumulate mcl-PHA in a range from 2.07 to 21.40%, which depended on the substrate used. Potential nitrogen-dependent regulation of mcl-PHA synthesis was analyzed by cell cultivation in nitrogen-limiting and non-limiting conditions. The analyzed strains demonstrated an incremental increase of mcl-PHAs in response to nitrogen starvation when oleic acid and sodium gluconate were applied as the carbon source. The transcriptional analysis showed that the induction of gene coding for PHA synthases was correlated with an increment in mcl-PHAs content. Both analyzed strains revealed differences in terms of the studied gene's expression, showing a dependence on the carbon source used.