Evaluation of the discriminatory powers of the dienes test and ribotyping as typing methods for Proteus mirabilis
Evaluation of the discriminatory powers of the dienes test and ribotyping as typing methods for Proteus mirabilis
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DOI：
10.1128/jcm.38.3.1077-1080.2000
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发表时间：
2000-03-01
影响因子：
9.4
通讯作者：
Doern, GV
中科院分区：
文献类型：
--
作者：
Pfaller, MA;Mujeeb, I;Doern, GV
通过 Dienes 测试和核糖分型对 19 个月内收集的总共 63 个奇异变形杆菌临床分离株进行了分型，核糖分型是使用全自动 RiboPrinter 微生物表征系统（Qualicon，威尔明顿，特拉华州）进行的。通过脉冲场凝胶电泳 (PFGE) 进一步表征通过二烯测试和/或核糖分型无法区分的分离株。根据 Dienes 测试和核糖分型判断，大多数分离株代表独特的菌株。四十个分离株代表了 40 种不同的核糖型和二烯类型。其余 23 个分离株分为 13 个二烯类型、12 个核糖类型和 14 个 PFGE 类型。 Dienes 测试的辨别指数为 0.980，核糖分型的辨别指数为 0.979，PFGE 的辨别指数为 0.992。 Dienes 测试和核糖分型都是鉴定奇异果单个菌株的有用方法。Dienes 测试简单、便宜且易于执行，几乎可以在任何实验室进行，并且应用于奇异果分离株的初始流行病学特征分析。
A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping, Ribotyping was performed using the fully automated RiboPrinter Microbial Characterization System (Qualicon, Wilmington, Del.). Isolates that were indistinguishable by the Dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (PFGE). Most of the isolates represented unique strains as judged by the Dienes test and ribotyping. Forty isolates represented 40 different ribotypes and Dienes types. The remaining 23 isolates were grouped into 13 Dienes types, 12 ribotypes, and 14 PFGE types. The index of discrimination was 0.980 for the Dienes test, 0.979 for ribotyping, and 0.992 for PFGE. Both the Dienes test and ribotyping are useful methods for identifying individual strains of P. mirabilis, The Dienes test is simple, inexpensive, and easy to perform, It can be performed in virtually any laboratory and should be used in the initial epidemiologic characterization of P. mirabilis isolates.