Water Quality and Diversity of Denitrifier Community Structure of Typical Scenic Water Bodies in Xi'an
Water Quality and Diversity of Denitrifier Community Structure of Typical Scenic Water Bodies in Xi'an
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DOI：
10.13227/j.hjkx.201703179
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发表时间：
2017-12-15
期刊：
影响因子：
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通讯作者：
Jia Jing-yu
中科院分区：
文献类型：
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作者：
Fang Peng-liang;Huang Ting-lin;Jia Jing-yu
采用Illumina高通量测序技术，选取6个微污染景观水体，探讨水质与nirs型反硝化菌群落结构和多样性的关系。水体理化分析结果表明，丰庆公园（FQ）和老东公园（LD）的水体为劣水体。曲江公园（QJ）、木塔西公园（MTS）和新集园公园（XJY）的水体被划分为？永阳公园（YY）水质为IV级，TN值为1.21 mg。L-1至6.50毫克。L-1，其中YY最低，FQ最高（P < 0.05）。TP显著高于LD （0.10 mg）。L-1)， QJ显著降低[0.02 mg]。L-1 (p < 0.05)]。LD的NH4+-N是QJ和FQ的4.44倍（P < 0.01）。Illumina高通量测序结果显示，6个水体反硝化菌群落组成差异显著，以副球菌（Paracoccus sp.）、假单胞菌（Pseudomonas sp.）和Rubrivivax sp.为优势属。主成分分析（PCA）表明，XJY和MTS的nirs型反硝化菌群落主要受NH4+-N调控，高锰酸盐指数、LD和FQ受NO3——N、NO2——N、TN、TP和DO的显著影响，YY受pH值的显著影响。结果表明，nirs型反硝化菌群落受不同水质指标的调控。
Six micro-polluted landscape water bodies were selected to explore the relationship between water quality and the nirS-Type denitrifier community structure and diversity with an Illumina high-throughput sequencing technique. The results of a physico-chemical analysis of the water bodies showed that the Fengqing Park (FQ) and Laodong Park (LD) water bodies were classified as inferior ? water quality, the Qujiang Park (QJ), Mutasi Park (MTS), and Xinjiyuan Park (XJY) water bodies were classified as ? water quality, and the Yongyang Park (YY) water quality was classified as IV. The TN values varied from 1.21 mg.L-1 to 6.50 mg.L-1, with the lowest value found in YY and the highest in FQ (P < 0.05). TP was significantly higher in LD (0.10 mg.L-1) and significantly lower in QJ[0.02 mg.L-1 (P < 0.05)]. The NH4+-N in LD was 4.44 times higher than that in QJ and FQ (P < 0.01). Illumina high-throughput sequencing revealed that the denitrifier community composition was significantly different among the six water bodies, and Paracoccus sp., Pseudomonas sp., and Rubrivivax sp. were the dominant genus species. A principle component analysis (PCA) indicated that the nirS-Type denitrifier communities of XJY and MTS were mainly regulated by NH4+-N and the permanganate index, LD and FQ were significantly influenced by NO3--N, NO2--N, TN, TP, and DO, and YY was significantly influenced by pH value. Our results showed that nirS-Type denitrifier communities were regulated by different water quality indicators.