Core-shell magnetic molecularly imprinted polymers: nanoparticles targeting selective androgen receptor modulators (sarms) and steroidal models
Core-shell magnetic molecularly imprinted polymers: nanoparticles targeting selective androgen receptor modulators (sarms) and steroidal models
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DOI：
10.1088/2632-959x/acce52
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发表时间：
2023-04
期刊：
影响因子：
3
通讯作者：
Mark V. Sullivan;B. Dean;Aiden Mates;Maria Elizabeth Farrow;Connor Fletcher;Maddie German;Riya M Patel;N. Turner
中科院分区：
文献类型：
--
作者：
Mark V. Sullivan;B. Dean;Aiden Mates;Maria Elizabeth Farrow;Connor Fletcher;Maddie German;Riya M Patel;N. Turner
以4-乙烯基吡啶为功能单体，三甲基丙烯酸异丙酯（TRIM）为交联剂，合成了粒径约为12 nm的超顺磁性氧化铁纳米粒子（SPION），使其成为印迹聚合物（MIP）壳层的磁性核，其平均粒径达到约45 nm。五个目标是imprinted-the选择性雄激素受体调节剂（SARM）andarine，ligandrol和RAD-140;和类固醇雌二醇和孕三烯酮。当加载非靶分子时，所有MMIP均产生良好的选择性，所有计算的选择性因子均高于1.2的推荐阈值，并且还表现出良好的亲和力/能力。还通过使用加标河水样品探索了目标分子从复杂基质中的重新结合。基于SARMs的MMIP能够重新结合99.56%、87.63%和72.78%的它们的靶分子（分别为Andarine、ligandrol和RAD-140），而基于类固醇的MMIP能够重新结合64.54%和55.53%的它们的靶分子（分别为雌二醇和孕三烯酮），其标称载量为50 mg NP中20 μg。这项工作突出了这些双功能材料用于复杂样品的痕量材料清理和/或后续分析的潜力，并为进一步简单，快速合成用于目标清理的材料开辟了可能性。
Super paramagnetic iron oxide nanoparticles (SPIONs) (∼12 nm) were synthesized as the magnetic core for an imprinted polymer (MIP) shell using 4-vinylpyridine as the functional monomer and trimethylolpropane trimethacrylate (TRIM) as the cross-linker, bringing the average size up to ∼45 nm. Five targets were imprinted—the Selective Androgen Receptor Modulators (SARMs) andarine, ligandrol and RAD-140; and the steroids estradiol and gestrinone. All MMIPs produced good selectivity when loaded with a non-target molecule, with all calculated selectivity factors above the 1.2 recommended threshold and also demonstrated good affinity/capacity. The rebinding of the target molecules from a complex matrix was also explored by using spiked river water samples. The SARMs-based MMIPs were able to rebind 99.56, 87.63 and 72.78% of their target molecules (andarine, ligandrol and RAD-140, respectively), while the steroidal-based MMIPs were able to rebind 64.54 and 55.53% of their target molecules (estradiol and gestrinone, respectively) at a nominal loading of 20 ≈μg in 50 mg of NPs. This work highlights the potential of these bi-functional materials for trace material clean-up of complex samples and/or subsequent analysis and opens up possibilities for further simple, rapid-to-synthesise materials for targeted clean-up.