Different organ and tissue tropism between Akabane virus genogroups in a mouse model
Different organ and tissue tropism between Akabane virus genogroups in a mouse model
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DOI：
10.1016/j.virusres.2022.198752
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发表时间：
2022-03-26
期刊：
影响因子：
5
通讯作者：
Horimoto, Taisuke
中科院分区：
文献类型：
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作者：
Takenaka-Uema, Akiko;Matsugo, Hiromichi;Horimoto, Taisuke
Akabane病毒（AKAV）是一种对家养反刍动物胎儿具有致畸性的病原体，导致牲畜繁殖能力的严重丧失。在东亚，AKAV分离株形成两个主要簇：基因群I和基因群II。近年来，基因组I分离株也与产后脑脊髓炎有关，主要发生在犊牛中。在此，我们比较了基因I组Iriki和基因II组OBE-1菌株对小鼠的致病性。只有腹腔感染Iriki菌株的小鼠死亡，并在中枢神经系统（CNS）和淋巴组织中表现出明显的复制。在临床阶段，iriki感染小鼠的血脑屏障（BBB）通透性升高，表明血脑屏障可能是病毒从外周传播到中枢神经系统的可能途径。这些结果表明，与OBE-1株相比，Iriki株具有更强的神经毒力和神经侵袭性，从而确定了不同基因组间AKAV的不同致病性。
Akabane virus (AKAV) is an etiological agent that is teratogenic to the fetus of domestic ruminants, causing a significant loss of reproduction in livestock. In East Asia, AKAV isolates form two major clusters: genogroups I and II. In recent years, genogroup I isolates have also been associated with postnatal encephalomyelitis, mainly in calves. Here, we compared the pathogenicity in mice using genogroup I Iriki and genogroup II OBE-1 strains. Only mice infected intraperitoneally with the Iriki strain died and showed marked replication in the central nervous system (CNS) and lymphoid tissues. A more elevated blood-brain barrier (BBB) permeability was found in the Iriki-infected mice in the clinical phase, indicating that the BBB might be a possible route of viral transmission from the periphery to the CNS. These findings demonstrate that the Iriki strain presents greater neurovirulence and neuroinvasiveness compared with the OBE-1 strain, determining different AKAV pathogenicity among genogroups.