Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages
Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages
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DOI：
10.1016/j.bbrc.2006.10.179
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发表时间：
2007-01-05
影响因子：
3.1
通讯作者：
Taniguchi, Hideki
中科院分区：
文献类型：
--
作者：
Zhao, Wenping;Hirose, Tomonori;Taniguchi, Hideki
胰岛新生的研究表明，胰岛细胞的再生可以通过预先存在的胰岛细胞的再分化来实现。然而，这一假说在很大程度上是未经证实的，并未能解释观察到的胰岛新生的多样性。在这里，我们表明，培养的新生胰腺细胞去分化成β III微管蛋白表达的前体，然后扩大和再分化成神经和胰腺谱系后代。再分化不仅发生在胰岛细胞中，也发生在导管细胞中，其可能代表所谓的导管来源的“胰腺干细胞”。它？新生胰腺细胞的体外再分化通过连续的内分泌分化来重现胚胎发育，所述内分泌分化伴随着神经元标记物β III微管蛋白与内分泌激素的共表达，直到终末分化。然而，胰腺细胞的神经元分化发生在内分泌分化之前，并逐渐占主导地位，因此意味着培养的胰腺细胞具有默认的神经元谱系特化。(c)2006年爱思唯尔公司All rights reserved.
Studies of islet neogenesis have suggested that the regeneration of islet cells can be achieved through redifferentiation of pre-existing islet cells. However, this hypothesis is largely unproven and fails to account for the diversity of observed islet neogenesis. Here we show that cultured neonatal pancreatic cells dedifferentiate into beta III tubulin-expressing precursors, which then expand and redifferentiate into both neural and pancreatic lineage progenies. Redifferentiation happens not only in the islet cells, but also in the ductal cells that may represent what are called ductal origin "pancreatic stem cells". The it? vitro redifferentiation of neonatal pancreatic cells recapitulates the embryonic development by sequential endocrine differentiation accompanied by the coexpression of neuronal marker beta III tubulin with endocrine hormones until terminal differentiation. The neuronal differentiation of pancreatic cells, however, occurs prior to endocrine differentiation and gradually becomes dominant, thus implying a default neuronal lineage specification for cultured pancreatic cells. (c) 2006 Elsevier Inc. All rights reserved.