Generation of a conditional Ppap2b/Lpp3 null allele
Generation of a conditional Ppap2b/Lpp3 null allele
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DOI：
10.1002/dvg.20314
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发表时间：
2007-07-01
期刊：
影响因子：
1.5
通讯作者：
Stewart, Colin L.
中科院分区：
文献类型：
--
作者：
Escalante-Alcalde, Diana;Sanchez-Sanchez, Roberto;Stewart, Colin L.
Lpp 3，以前称为Pap 2b，是一种脂质磷酸水解酶。它的一些底物和产物是具有有效生物和信号传导活性的脂质，如磷脂酸、溶血磷脂酸、鞘氨醇-1-磷酸、二酰基甘油、鞘氨醇和神经酰胺。Lpp 3在发育过程中动态表达，并广泛分布于成体组织中。Lpp 3基因（Ppap 2b）的靶向失活导致小鼠胚胎死亡，因为胚胎外血管发育和原肠胚形成的缺陷。为了研究Lpp 3在发育后期和特定细胞谱系中的参与，我们产生了Ppap 2b的条件等位基因。这是通过侧翼的关键外显子，负责其催化活性与loxP网站。一个广义的Cre-mediated重组这个等位基因产生的表型基本相同，我们以前报道的Ppap 2b无效等位基因。
Lpp3, formerly known as Pap2b, is a lipid phosphohydrolase enzyme. Some of its substrates and products are lipids with potent biological and signaling activities such as phosphatidic acid, lysophosphatidic acid, sphingosine-1-phosphate, diacylglycerol, sphingosine, and ceramide. Lpp3 is dynamically expressed during development and is widely distributed in adult tissues. Targeted inactivation of Lpp3 gene (Ppap2b) in the mouse results in embryonic lethality because of defects in extraembryonic vascular development and gastrulation. To study the participation of Lpp3 later in development and in specific cell lineages we generated a conditional allele of Ppap2b. This was accomplished by flanking critical exons, responsible for its catalytic activity with loxP sites. A generalized Cre-mediated recombination of this allele yielded a phenotype fundamentally identical to our previously reported Ppap2b null allele.