Gene inactivation in the oral spirochete Treponema denticola: Construction of an flgE mutant
Gene inactivation in the oral spirochete Treponema denticola: Construction of an flgE mutant
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DOI：
10.1128/jb.178.12.3664-3667.1996
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发表时间：
1996-06-01
影响因子：
3.2
通讯作者：
Kuramitsu, H
中科院分区：
文献类型：
--
作者：
Li, H;Ruby, J;Kuramitsu, H
齿垢密螺旋体与牙周病的病因有关。用插入flgE DNA片段中的红霉素抗性盒电穿孔后，将重组质粒转染齿垢菌ATCC 35405。所得的突变体显示没有可见的运动性和杰克周质鞭毛将从鞭毛钩蛋白基因的失活预测。
Treponema denticola is implicated in the etiology of periodontal diseases, We now report the construction of a specific flgE mutant of T. denticola ATCC 35405 following electroporation utilizing an erythromycin resistance cassette inserted into an flgE DNA fragment. The resulting mutant displays no visible motility and Jacks periplasmic flagella as would be predicted from inactivation of the gene for the flagellar hook protein.