Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka
Production of Wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column in medaka
复制标题
DOI：
10.1242/dev.051540
复制
发表时间：
2010-06-01
期刊：
影响因子：
4.6
通讯作者：
Kudo, Akira
中科院分区：
文献类型：
--
作者：
Inohaya, Keiji;Takano, Yoshiro;Kudo, Akira
底板是一个关键的组织者，控制着中枢神经系统中神经元的规格。在这里，我们展示了底板的一个新作用：脊柱的节段性模式形成。对青竹自发性突变体融合椎体(FSC)的分析表明，FSC编码wnt4b，它只在底板中表达。在FSC突变体中，我们发现wnt4b在底板中的表达完全缺失，椎间韧带细胞向成骨细胞的异常转化似乎导致了椎间韧带的缺陷。转基因救援系的建立和镶嵌分析的建立使得我们可以得出结论，底板细胞产生wnt4b对于脊柱的节段性模式是必不可少的。我们的发现为脊椎动物发育的机制提供了一个新的视角。
The floor plate is a key organizer that controls the specification of neurons in the central nervous system. Here, we show a new role of the floor plate: segmental pattern formation of the vertebral column. Analysis of a spontaneous medaka mutant, fused centrum (fsc), which exhibits fused centra and the absence of the intervertebral ligaments, revealed that fsc encodes wnt4b, which was expressed exclusively in the floor plate. In fsc mutants, we found that wnt4b expression was completely lost in the floor plate and that abnormal conversion of the intervertebral ligament cells into osteoblasts appeared to cause a defect of the intervertebral ligaments. The establishment of the transgenic rescue lines and mosaic analyses allowed the conclusion to be drawn that production of wnt4b by floor plate cells is essential for the segmental patterning of the vertebral column. Our findings provide a novel perspective on the mechanism of vertebrate development.