Multiple vitellogenin-derived yolk proteins in gray mullet (Mugil cephalus):: Disparate proteolytic patterns associated with ovarian follicle maturation
Multiple vitellogenin-derived yolk proteins in gray mullet (Mugil cephalus):: Disparate proteolytic patterns associated with ovarian follicle maturation
复制标题
DOI：
10.1002/mrd.20864
复制
发表时间：
2008-08-01
影响因子：
2.5
通讯作者：
Hara, Akihiko
中科院分区：
文献类型：
--
作者：
Amano, Haruna;Fujita, Toshiaki;Hara, Akihiko
在灰鲻鱼中观察到源自三种卵黄原蛋白（VgA、VgB 和 VgC）的卵黄蛋白的不同蛋白水解模式。对从卵黄发生卵巢 (NO) 和排卵卵 (OE) 中获得的提取物进行的免疫生化分析证实，在卵巢卵泡成熟过程中，大部分 VgA 衍生的卵黄蛋白 (LvA) 被降解为游离氨基酸 (FAA)。 VgB 衍生的 Lv (LvB) 的成熟相关改变仅涉及有限的蛋白水解作用； LvB重链和轻链分别解离成至少三个和一个多肽片段。 VgC 衍生的 Lv (LvC) 的天然质量在 VO 和 OE 之间几乎没有差异，尽管很明显 LvC 在成熟过程中被“切口”，导致 OE 中出现几个条带。类似的分析证实，VgA 衍生的 β'-组分 (β'-cA) 和 VgB 衍生的 β'-c (β'-cB) 在成熟过程中数量和天然质量均下降，而源自 VgA (PvA) 或 VgB (PvB) 的卵黄高磷蛋白似乎降解为 FAA。鲻鱼卵黄蛋白的成熟相关蛋白水解模式与其他海洋硬骨鱼产卵远洋卵的报道相似。然而，鲻鱼 VO 中三种不同类型 Lv 的沉积率似乎与另一种海洋鱼类鲽鱼的估计值不同。这些结果支持了最近的一个范式，即 Vg 多样性对这组鱼类中连续生理事件的重要性，包括成熟卵母细胞的水合、获得适当的卵浮力以及胚胎发生和幼体发育每个阶段所需营养储备的产生。
Disparate proteolytic patterns of yolk proteins, derived from three types of vitellogenin (VgA, VgB, and VgC), were observed in gray mullet. Immuno-biochemical analyses of extracts obtained from vitellogenic ovaries NO) and ovulated eggs (OE) confirmed that a large proportion of VgA-derived lipovitellin (LvA) was degraded into free amino acids (FAAs) during ovarian follicle maturation. The maturation-associated alteration of VgB-derived Lv (LvB) involved only limited proteolysis; the heavy and light LvB chains were dissociated into at least three and one polypeptide fragments, respectively. The native mass of VgC-derived Lv (LvC) exhibited little difference between VO and OE, although it was apparent that the LvC was 'nicked' during maturation, resulting in the appearance of several bands in OE. Similar analyses confirmed that VgA-derived beta'-component (beta'-cA) and VgB-derived beta'-c (beta'-cB) decreased during maturation in both quantity and native mass, while phosvitin derived from either VgA (PvA) or VgB (PvB) appeared to be degraded into FAAs. The pattern of maturation-associated proteolysis of mullet yolk proteins is similar to that reported for other marine teleosts spawning pelagic eggs. However, the depository ratio of the three distinct types of Lv in the mullet VO appeared to be different from that estimated for another marine pelagophil, the barfin flounder. These results support a recent paradigm regarding the significance of Vg multiplicity upon successive physiological events in this group of fishes including the hydration of maturing oocytes, the acquisition of proper egg buoyancy, and the generation of requisite nutrient stocks for each stage of embryogenesis and larval development.