Time-dependent responses to glp-1-mediated inductions in early C. elegans embryos.
Time-dependent responses to glp-1-mediated inductions in early C. elegans embryos.
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发表时间：
1996-07
期刊：
影响因子：
4.6
通讯作者：
C. A. Shelton;B. Bowerman
中科院分区：
文献类型：
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作者：
C. A. Shelton;B. Bowerman
在秀丽隐杆线虫的胚胎中，两个卵裂球在4-细胞阶段，阿坝和ABp，出生时具有相同的发育潜力。随后，在4-细胞阶段与P2卵裂球和在12-细胞阶段与MS卵裂球的相互作用在阿坝和ABp的后代之间产生发育命运的差异。我们已经复制了这些诱导在体外细胞培养基中分离的胚胎卵裂球。我们表明，在这些诱导过程中，只有响应AB后代需要GLP-1基因的活性，这是类似的序列果蝇Notch，支持模型中，GLP-1蛋白作为受体的P2和MS信号。我们还表明，P2信号需要的apx-1基因的活性，类似的序列果蝇三角洲，和MS信号需要假定的转录因子SKN-1。我们提出的证据表明，决定这两个信号的不同反应的主要因素是AB后代的年龄，而不是信号细胞或配体的身份。因此，我们认为，AB后代的因素随时间的变化是负责他们的不同反应的诱导信号，使用一个共同的受体。
In an embryo of the nematode Caenorhabditis elegans, two blastomeres at the 4-cell stage, ABa and ABp, are born with equivalent developmental potential. Subsequently, interactions with the P2 blastomere at the 4-cell stage and the MS blastomere at the 12-cell stage generate differences in developmental fate among descendants of ABa and ABp. We have reproduced these inductions in vitro using embryonic blastomeres isolated in cell-culture medium. We show that during these inductions only the responding AB descendants require the activity of the glp-1 gene, which is similar in sequence to Drosophila Notch, supporting models in which GLP-1 protein acts as a receptor for both the P2 and MS signals. We also show that P2 signaling requires the activity of the apx-1 gene, similar in sequence to Drosophila Delta, and that MS signaling requires the putative transcription factor SKN-1. We present evidence that the primary factor determining the different responses to these two signals is the age of the AB descendants, not the identity of the signaling cell or ligand. Therefore, we suggest that time-dependent changes in factors within AB descendants are responsible for their different responses to inductive signals that use a common receptor.