The subcellular localization of a C-terminal processing protease in Pseudomonas aeruginosa
The subcellular localization of a C-terminal processing protease in Pseudomonas aeruginosa
复制标题
DOI：
10.1111/j.1574-6968.2010.02181.x
复制
发表时间：
2011-03-01
影响因子：
2.1
通讯作者：
Rosenau, Frank
中科院分区：
文献类型：
--
作者：
Hoge, Rien;Laschinski, Marko;Rosenau, Frank
羧基末端加工酶(CTP)是一类相对较新的丝氨酸蛋白酶。这些蛋白水解酶广泛存在于细菌、古菌、藻类、植物和动物等生物体内，参与蛋白质的C末端加工。与细菌蛋白质的氨基末端加工相比，C末端加工及其生理功能知之甚少。细菌CTP似乎影响不同的基本细胞过程。尽管革兰氏阴性菌的CTP通常被认为定位于周质，但几乎没有实验证据证明这一点。我们通过精心设计的分级研究首次显示了来自铜绿假单胞菌的CTP-3家族蛋白CTPA在周质中的亚细胞定位。我们的结果为普遍接受的假设提供了实验证据，即CTPs位于革兰氏阴性细菌的周质空间。
Carboxy (C)-terminal processing proteases (CTP) are a relatively new group of serine proteases. Found in a broad range of organisms - bacteria, archaea, algae, plants and animals - these proteases are involved in the C-terminal processing of proteins. In comparison with amino-terminal processing of bacterial proteins, less is known about C-terminal processing and its physiological function. Bacterial CTPs appear to influence different basal cellular processes. Although CTPs of Gram-negative bacteria are generally referred to as being localized in the periplasm, there is little experimental evidence for this. We show for the first time the subcellular localization of a CTP-3 family protein from Pseudomonas aeruginosa, named CtpA, in the periplasm by a carefully designed fractionation study. Our results provide experimental evidence for the generally accepted hypothesis that CTPs are located in the periplasmic space of Gram-negative bacteria.