Impact of extremely low frequency electromagnetic fields on CD4 expression in peripheral blood mononuclear cells
Impact of extremely low frequency electromagnetic fields on CD4 expression in peripheral blood mononuclear cells
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DOI：
10.1023/a:1007004316433
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发表时间：
1999-11-01
影响因子：
4.3
通讯作者：
Conti, P
中科院分区：
文献类型：
--
作者：
Felaco, M;Reale, M;Conti, P
越来越多的证据表明，极低频电磁场（ELF-EMF）可能会影响多种细胞功能。本文应用荧光激活细胞分选仪（FACScan）研究了极低频电磁场（ELF-EMF）对人外周血单个核细胞（PBMC）CD 4（+）细胞表面受体表达的影响。ELF-EMF暴露24、48和72 h与未暴露PBMC中CD 4（+）的表达差异无统计学意义。此外，在24和72 h孵育时，通过使用荧光标记抗体测定流式细胞术分析。与未暴露的细胞相比，暴露于ELF-EMF的PBMC中结合抗体的量分布略有差异。此外，PBMC中的DNA CD 4（+）表达在暴露的细胞中强烈增加，静息和用植物血凝素（PHA）激活。用聚合酶链反应（PCR）检测PBMC中CD 4（+）mRNA的表达，发现ELF-EMF作用24 h后，静止细胞中CD 4（+）mRNA的表达较未作用细胞增加，而48 h和72 h时无差异。与未暴露的细胞相比，暴露于ELF-EMF的PBMC呈现S期细胞周期进展的百分比表达的显著增加;而G1期和G2期则无差异。我们的研究结果提供了新的证据表明ELF-EMF可以影响PBMC中的CD 4（+）表达，并描述了ELF-EMF影响CD 4（+）转录和翻译蛋白以及增加S期细胞周期进程的百分比表达的额外生物活性。
There is increasing evidence suggesting that extremely low frequency electromagnetic fields (ELF-EMF) may influence several cell functions. Here the effects of ELF-EMF were studied on the expression of CD4(+) cell surface receptors of human peripheral blood mononuclear cells (PBMC) using florescence-activated cell sorter (FACScan). The expression of CD4(+) in ELF-EMF exposed (24, 48 and 72 h) and not exposed PBMC were not statistically significant. In addition, a flow cytometric analysis was determined by using a fluorescent labeled antibody, at 24 and 72 h incubations. The amount of bound antibody was distributed with a slight difference in the ELF-EMF-exposed PBMC compared to the not exposed cells. Moreover, DNA CD4(+) expression in PBMC strongly increased in exposed cells, resting and activated with Phytohaemaglutinin (PHA). When polymerase chain reaction was performed on CD4(+) mRNA of PBMC an increase of CD4(+) mRNA expression was found after the resting cells were exposed to ELF-EMF at 24 h compared to not exposed cells, while at 48 and 72 h no difference was found. In the cell cycle progression analysis, the PBMC exposed to ELF-EMF presented a significant increase of percentage expression of cell cycle progression in the S phase compared to not exposed cells; while in G1 and G2 phases, there were no differences. Our results provide new evidence that ELF-EMF can affect CD4(+) expression in PBMC and describe an additional biological activity for ELF-EMF affecting CD4(+) transcription and translation protein and the increase of the percentage expression of the cell cycle progression of the S phase.