The PR10 gene family is highly expressed in Lilium regale Wilson during Fusarium oxysporum f. sp lilii infection
The PR10 gene family is highly expressed in Lilium regale Wilson during Fusarium oxysporum f. sp lilii infection
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PR10 基因家族在尖孢镰刀菌 (Fusarium oxysporum f) 期间在百合 (Lilium regale Wilson) 中高度表达。
DOI：
10.1007/s13258-014-0185-x
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发表时间：
2014-08-01
期刊：
影响因子：
2.1
通讯作者：
Chen, Chaoyin
中科院分区：
文献类型：
--
作者：
He, Hua;Liu, Diqiu;Chen, Chaoyin
发病机制相关（PR）蛋白在植物对病原体和非生物胁迫的反应中发挥着关键作用。本研究从中国野生百合品种百合中分离出 9 个新的 PR 基因，对土传真菌尖镰孢具有高水平的抗性。 sp。 lilii，同源分析将其归入PR10家族。这些新的LrPR10与来自单子叶植物的PR10在系统发育树中聚集在一起，而且系统发育分析将9个LrPR10分为两组。 LrPR10 的主链构象和折叠模式与其他植物 PR10 高度保守。通过QRT-PCR检测了富贵鱼正常发育过程中9个LrPR10的表达模式，发现LrPR10在根中的转录水平相对较高。此外，QRT-PCR分析表明，富贵花根中LrPR10-1、LrPR10-2、LrPR10-5、LrPR10-6和LrPR10-7的表达水平受到水杨酸、茉莉酸、乙烯和H2O2等两种或多种胁迫相关信号分子的上调，而其他四个LrPR10则受到这四种信号分子的抑制。 分子。此外，与易感东方百合“西伯利亚”相比，抗性百合中的 LrPR10 基因家族的 5 个成员（包括 LrPR10-2、LrPR10-4、LrPR10-5、LrPR10-6、LrPR10-7 和 LrPR10-9）受到尖孢镰刀菌的强烈诱导。其他四个 LrPR10 因尖孢镰刀菌感染而下调。总之，我们的结果表明 PR10 基因家族的成员参与了 L. regale 针对 F. oxysporum f 的防御反应。 sp。莉莉。
Pathogenesis-related (PR) proteins play key roles in plant responses to pathogens and abiotic stresses. In this study, nine novel PR genes were isolated from Lilium regale Wilson, which is a wild lily species of China with high-level resistance to the soilborne fungal pathogen Fusarium oxysporum f. sp. lilii, and homology analysis classified them into the PR10 family. These novel LrPR10s were clustered together with PR10s from monocotyledons in a phylogenetic tree, moreover, phylogenetic analysis divided the nine LrPR10s into two groups. The main-chain conformation and folding patterns of the LrPR10s were highly conserved with other plant PR10s. The expression patterns of the nine LrPR10s in L. regale during normal development were examined by QRT-PCR, and the transcription levels of the LrPR10s were relatively high in roots. Furthermore, QRT-PCR analysis indicated that the expression levels of LrPR10-1, LrPR10-2, LrPR10-5, LrPR10-6, and LrPR10-7 in L. regale roots were up-regulated by two or more stress-related signaling molecules including salicylic acid, jasmonic acid, ethylene, and H2O2, while the other four LrPR10s were repressed by these four signaling molecules. In addition, five members of the LrPR10 gene family including LrPR10-2, LrPR10-4, LrPR10-5, LrPR10-6, LrPR10-7, and LrPR10-9 were strongly induced by F. oxysporum in resistant L. regale compared with the susceptible Lilium Oriental hybrid ‘Siberia’. The other four LrPR10s were down-regulated by F. oxysporum infection. In summary, our results indicate that the members of PR10 gene family are involved in L. regale defense responses against F. oxysporum f. sp. lilii.