Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.
Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.
复制标题
人和兔 IL-1 前体 cDNA 的克隆和表征。
作者：
Y. Furutani;M. Notake;M. Yamayoshi;J. Yamagishi;H. Nomura;M. Ohue;R. Furuta;T. Fukui;M. Yamada;S. Nakamura
利用部分纯化的poly(A)+RNA从兔肺泡巨噬细胞中克隆cDNA文库，通过mRNA杂交-翻译法克隆出兔白细胞介素-1前体（preIL-1） mRNA的互补DNA序列。以该cDNA为探针，从人单核细胞样细胞系HL-60细胞诱导的poly(a)+RNA cDNA文库中分离到人IL-1 cDNA。从cDNA序列中推断出人和家兔preIL-1的氨基酸序列，分别由271和267个氨基酸残基组成。该氨基酸序列在人和兔之间保守性为64%。氨基酸残基数目的差异是由人preIL-1中4个氨基酸残基的羧基末端延伸引起的。克隆的人cDNA在大肠杆菌中表达得到具有生物活性的IL-1。
DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.