Konjaku flour reduces obesity in mice by modulating the composition of the gut microbiota
Konjaku flour reduces obesity in mice by modulating the composition of the gut microbiota
复制标题
DOI：
10.1038/s41366-018-0187-x
复制
发表时间：
2019-08-01
影响因子：
4.9
通讯作者：
Kong, Xiangyang
中科院分区：
文献类型：
--
作者：
Kang, Yongbo;Li, Yu;Kong, Xiangyang
研究背景肠道植物群组成的改变被称为肠道生态失调，它与肥胖的发生发展有关，因此支持营养物质作用于肠道植物群对宿主能量代谢发挥有益作用的潜在作用。已知膳食纤维影响肠道植物群的组成。本研究旨在探讨玉米粉（KF）在改善炎症、代谢和肠道屏障功能方面对肥胖控制的作用，以及其作用与肠道植物群组成变化的可能关系。方法小鼠（n = 30）随机分为对照组（n = 10）、高脂饮食组（n = 10）、KF干预组（n = 10），喂养12周后，治疗组在此基础上每日添加KF。结果KF能显著降低HFD小鼠体重、脂肪量和脂肪细胞大小的增加，并降低血清TC、瘦素（LEP）、硫代巴比妥酸反应物质（TBARS）、IL-6、IL-10、IL-11、IL-12、IL-14、IL-15、IL-16、IL-17、IL-18、IL-19和脂多糖（LPS）水平。KF还可上调HFD小鼠肠黏膜蛋白基因Intection和紧密连接ZO-1的表达，上调肝脏和脂肪组织中能量代谢基因PPAR α、CPT-1和脂肪代谢基因HLS的表达，下调脂肪合成基因PPAR γ的表达（p < 0.05）。KF处理增加了HFD小鼠肠道菌群的α多样性并改变了HFD小鼠肠道菌群的n多样性，并且增加了HFD小鼠肠道菌群中一些与肥胖相关的有益微生物（如埃氏巨球菌）的丰度，而减少了有害微生物（如Alistipes，Alloprevotella，Bacteroides acidifaciens和Parabacteroides goldsteinii）的丰度。Alistipes的丰度与体重、脂肪量、血清TC、TG、LEP、IL-6、LPS含量及PPAR γ基因表达呈显著正相关，而与CPT-1、HLS基因表达呈显著负相关（p < 0.01）。结论KF可通过调节肠道菌群结构，改善HFD喂养小鼠的炎症反应、代谢和肠道屏障功能，从而达到治疗肥胖的作用。
Background Changes in the intestinal flora composition is referred to as dysbiosis, which is related to obesity development, thus supporting the potential roles of nutrients acting on intestinal flora to exert salutary effects on energetic metabolism of host. Dietary fiber has been known to affect the composition of intestinal flora. The aim of the present study was to investigate the functional effects of konjac flour (KF) on obesity control in respect to improving inflammation, metabolism, and intestinal barrier function, and the possible association of the effects with intestinal flora composition changes.Methods Mice (n = 30) were randomly divided into control group (n = 10), high-fat-diet (HFD) group (n = 10), and KF intervention group (n = 10), followed by feeding for 12 weeks and with adding a KF daily supplementation for the treatment group. Body weight, fat accumulation, inflammation, and energetic metabolism markers in multiple tissues and the gut microbiota of the mice were examined at the end of the experiment.Results The KF supplementation significantly reduced the gains in weight, fat mass, as well as adipocyte size of HFD mice and lowered the serum TC, leptin (LEP), thiobarbituric acid-reacting substance (TBARS), IL-6, and lipopolysaccharide (LPS) levels in HFD mice. KF also upregulated the expression of intestinal mucosa protein gene Intection and tight junction ZO-1 in HFD mice, as well as upregulate the expression of energy metabolism genes PPAR alpha and CPT-1 as well as the fat metabolism gene HLS in livers and fat tissues, and downregulate that of fat synthesis gene PPAR gamma (p < 0.05). The KF treatment increases the a-diversity and change the n-diversity of the intestinal microflora in HFD mice and boosted the abundances of some obesity-related beneficial microorganisms (such as Megasphaera elsdenii) in the intestinal microflora of HFD mice, while reduced those of harmful microorganisms (such as Alistipes, Alloprevotella, Bacteroides acidifaciens, and Parabacteroides goldsteinii). The abundance of Alistipes was positively correlated with weight, fat mass, serum TC, TG, LEP, IL-6, and LPS contents as well as PPAR gamma gene expression; while notably and negatively related to the expression of CPT-1 and HLS genes (p < 0.01). KF remarkably increased the abundance of Aerococcaceae, while reduced that of Alistipes finegoldii (p < 0.01).Conclusions Supplementation with KF achieves favorable effects on treating obesity, improving inflammatory response, metabolism, and intestinal barrier function, by regulating intestinal microfloral structure in HFD-fed mice.