The genome project of Taenia solium
The genome project of Taenia solium
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DOI：
10.1016/j.parint.2005.11.020
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发表时间：
2006-01-01
影响因子：
1.9
通讯作者：
Yánez, J
中科院分区：
文献类型：
--
作者：
Aguilar-Díaz, H;Bobes, RJ;Yánez, J
我们在墨西哥国立自治大学组建了一个由重点实验室组成的联盟，以开展猪带绦虫基因组项目。该项目将为绦虫/囊虫病的研究提供强大的资源，并与细粒棘球绦虫和多房棘球绦虫表达序列标签（est）基因组计划相结合，标志着绦虫寄生虫基因组学的到来。我们的项目计划分为两个连续的阶段。第一阶段正在进行，以确定T solium基因组的一些基本参数。随后，我们将评估第二阶段的最佳策略，即完整的基因组计划。我们通过两种不同的方法估计了T solium基因组的大小：对分离的细胞核进行细胞荧光测定，以及基于类似于2000个测序基因组克隆的概率计算，类似于3000个ESTs，分别得出270和251 Mb的大小估计。在测序方面，我们第一阶段的目标是表征数千个EST（来自成虫和囊虫cDNA文库）和基因组克隆。到目前为止，从大约16000个成年期的est序列中获得的结果表明，只有大约40%的T虫编码序列具有先前测序的同源性。在哺乳动物的基因中发现了许多最受欢迎的基因，尤其是人类。然而，1.5%的被击中的基因在人类中缺乏同源物，使这些基因成为药物治疗、诊断和疫苗接种研究的直接候选基因。大多数T细胞est与基因调控和信号转导有关。其他重要的功能包括家政、代谢、细胞分裂、细胞骨架、蛋白酶、空泡运输、激素反应和细胞外基质活动。初步结果还表明，T solium的基因组不是高度重复的。2005爱思唯尔爱尔兰有限公司版权所有。
We have constituted a consortium of key laboratories at the National Autonomous University of Mexico to carry out a genomic project for Taenia solium. This project will provide powerful resources for the study of taeniasis/cysticercosis, and, in conjunction with the Echinococcus granulosus and Echinococcus multilocularis genome project of expressed sequence tags (ESTs), will mark the advent of genomics for cestode parasites. Our project is planned in two consecutive stages. The first stage is being carried out to determine some basic parameters of the T solium genome. Afterwards, we will evaluate the best strategy for the second stage, a full blown genome project, We have estimated the T solium genome size by two different approaches: cytofluorometry on isolated cyton nuclei, as well as a probabilistic calculation based on similar to 2000 sequenced genomic clones, similar to 3000 ESTs, resulting in size estimates of 270 and 251 Mb, respectively. In terms of sequencing, our goal for the first stage is to characterize several thousand EST's (from adult worm and cysticerci cDNA libraries) and genomic clones. Results obtained so far from about 16,000 sequenced ESTs from the adult stage, show that only about 40% of the T solium coding sequences have a previously sequenced homologue. Many of the best hits are found with mammalian genes, especially with humans. However, 1.5% of the hits lack homologues in humans, making these genes immediate candidates for investigation on pharmaco-therapy, diagnostics and vaccination. Most T solium ESTs are related to gene regulation, and signal transduction. Other important functions are housekeeping, metabolism, cell division, cytoskeleton, proteases, vacuolar transport, hormone response, and extracellular matrix activities. Preliminary results also suggest that the genome of T solium is not highly repetitive. (C) 2005 Elsevier Ireland Ltd. All rights reserved.