Identification of spinal neurons involved in the urethrogenital reflex in the female rat.
Identification of spinal neurons involved in the urethrogenital reflex in the female rat.
复制标题
鉴定参与雌性大鼠尿道生殖反射的脊髓神经元。
DOI：
10.1002/cne.10732
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Makhanova,Natalia
中科院分区：
文献类型：
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作者：
Marson,Lesley;Cai,Rong;Makhanova,Natalia
尿道生殖器反射（UG）是一种脊柱性反射，由自主神经和躯体神经活动以及阴道、子宫和肛门括约肌收缩组成。UG反射受到副巨细胞核（nPGi）区域神经元的紧张性下行抑制。在雌性大鼠中使用立即早期基因c-fos检查了由UG反射激活的脊髓神经元的位置。此外，使用注射到nPGi中的顺行示踪剂生物素葡聚糖胺检查从nPGi到fos激活神经元的下行输入。与未激活UG反射的实验对照相比，UG反射导致T12-S1节段中fos阳性核显著增加。参与UG反射的脊髓回路包括在L5-S1的背角和内侧脊髓中中继来自阴部感觉神经的传入信息的神经元。传出输出包括位于L5-S1外侧灰质和T13-L2外侧和内侧灰质的节前神经元。参与UG反射的脊髓中间神经元被发现接近节前神经元和背角和中间和内侧灰质的T12-S1。NPGi输入主要在内侧和中间灰质的自主传出神经和中间神经元上发现。这些研究表明，UG反射激活的多节段脊髓回路，并表明nPGi的下行抑制是通过节前和躯体传出和脊髓中间神经元密切相关的传出输出。神经学比较杂志462：355-370，2003.© 2003 Wiley利斯公司
The urethrogenital (UG) reflex is a spinal sexual reflex that consists of autonomic and somatic nerve activity and vaginal, uterine, and anal sphincter contractions. The UG reflex is under tonic descending inhibition by neurons in the region of the nucleus paragigantocellularis (nPGi). The location of spinal neurons activated by the UG reflex was examined in the female rat using the immediate early gene, c‐fos. In addition, the descending inputs from the nPGi onto fos‐activated neurons was examined using the anterograde tracer biotin dextran amine injected into the nPGi. The UG reflex resulted in a significant increase in fos‐positive nuclei in segments T12–S1, compared with experimental controls in which the UG reflex was not activated. Spinal circuits involved in the UG reflex include neurons relaying afferent information from the pudendal sensory nerve, in the dorsal horn and medial cord of L5–S1. Efferent output includes preganglionic neurons located in the lateral gray of L5–S1 and lateral and medial gray of T13–L2. Spinal interneurons involved in the UG reflex were found close to the preganglionic neurons and in the dorsal horn and intermediate and medial gray of T12–S1. NPGi inputs were found primarily on the autonomic efferents and interneurons in the medial and intermediate gray. These studies demonstrate multisegmental spinal circuits activated with the UG reflex and demonstrate that the descending inhibition from the nPGi is by means of preganglionic and somatic efferents and spinal interneurons closely associated with the efferent output. J. Comp. Neurol. 462:355–370, 2003. © 2003 Wiley‐Liss, Inc.