The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal.
The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal.
复制标题
DOI：
10.1016/j.pep.2011.04.011
复制
发表时间：
2011-08
影响因子：
1.6
通讯作者：
Wiener, Michael C.
中科院分区：
文献类型：
--
作者：
Vergis, James M.;Wiener, Michael C.
Recombinant proteins typically include one or more affinity tags to facilitate purification and/or detection. Expression constructs with affinity tags often include an engineered protease site for tag removal. Like other enzymes, the activities of proteases can be affected by buffer conditions. The buffers used for integral membrane proteins contain detergents, which are required to maintain protein solubility. We examined the detergent sensitivity of six commonly-used proteases (Enterokinase, Factor Xa, Human Rhinovirus 3C Protease, SUMOstar, Tobacco Etch Virus Protease, and Thrombin) by use of a panel of ninety-four individual detergents. Thrombin activity was insensitive to the entire panel of detergents, thus suggesting it as the optimal choice for use with membrane proteins. Enterokinase and Factor Xa were only affected by a small number of detergents, making them good choices as well.
登录
查看更多内容
影响因子：
8
作者：
Cabrita, Lisa D.;Gilis, Dimitri;Bottomley, Stephen P.
通讯作者：
Bottomley, Stephen P.
影响因子：
56.9
作者：
Shultis, David D.;Purdy, Michael D.;Wiener, Michael C.
通讯作者：
Wiener, Michael C.
影响因子：
2.9
作者：
Vergis, James M.;Purdy, Michael D.;Wiener, Michael C.
通讯作者：
Wiener, Michael C.
影响因子：
1.6
作者：
Mohanty, AK;Simmons, CR;Wiener, MC
通讯作者：
Wiener, MC
影响因子：
1.6
作者：
Sticha, KRK;Sieg, CA;Wagner, CR
通讯作者：
Wagner, CR