Two Proteins of Gene A of ϕX174
Two Proteins of Gene A of ϕX174
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φX174 基因 A 的两种蛋白质
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
M. Hayashi
中科院分区：
文献类型：
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作者：
E. Linney;M. Hayashi
单链DNA噬菌体X174和S13的基因A是在感染性单链DNA 1 -3上合成DNA的互补链后复制双链复制型（RF）所需的唯一噬菌体基因。它是一个顺式作用基因，基因A突变体和其他噬菌体基因中的突变体的双重感染未能拯救突变体A噬菌体4，这表明基因A产物只能在合成它们的基因组上发挥作用。基因A对于检测亲本RF 5中的顺式相关病毒链特异性不连续性是必需的。已经提出，在BAX 174基因组的基因A区域包含DNA复制的起源6，并且已经提出遗传学证据来支持基因A产生两种蛋白质A和A′（分子量分别为35，000和60- 65，000）的模型7。
GENE A of the single strand DNA phages ϕX174 and S13 is the only phage gene required for the replication of the double stranded replicative form (RF) after the complementary strand of DNA is synthesized upon the infecting single strand DNA1–3. It is a cis acting gene, double infection with gene A mutants and mutants in the other phage genes fail to rescue mutant A phage4, suggesting that gene A products can only function upon the genome from which they are synthesized. Gene A is necessary for the detection of a cis related viral strand specific discontinuity in the parental RF5. The gene A region of the ϕX174 genome has been suggested to contain the origin of DNA replication6 and genetic evidence has been presented to support the model that gene A produces two proteins, A and A′ (molecular weight 35,000 and 60–65,000, respectively)7.