Interactions of earthworms with indigenous and bioaugmented PCB-degrading bacteria
Interactions of earthworms with indigenous and bioaugmented PCB-degrading bacteria
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DOI：
10.1111/j.1574-6941.2002.tb00980.x
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发表时间：
2002-09-01
影响因子：
4.2
通讯作者：
Crowley, DE
中科院分区：
文献类型：
--
作者：
Luepromchai, E;Singer, AC;Crowley, DE
利用 PCB 降解细菌和蚯蚓进行生物强化，已实现了多氯联苯 (PCB) 污染土壤的部分生物修复。为了进一步研究蚯蚓对生物修复的贡献，我们进行了一项实验，分析了单独使用蚯蚓（地龙）或与多氯联苯降解菌、富养罗尔斯顿菌和红球菌联合处理污染土壤过程中本地和生物增强的多氯联苯降解菌的变化。 ACS。用于生物增强的细菌在培养物中用香芹酮和水杨酸诱导，并每 3-4 天重复施用到含有 100 ppm Aroclor 1242 的未混合、20 厘米长的土柱表面。处理 9 周后，使用 bph 基因的 PCR 引物分析土壤细菌群落。结果表明，使用蚯蚓和生物强化相结合，可以去除顶部 9 厘米处的约 50% 的 PCB，而单独使用生物强化或蚯蚓仅能有效去除土柱顶部 3 厘米处的 PCB。蚯蚓对 PCB 的去除增强与可培养的本地联苯降解细菌种群规模的增加以及 bphA 和 bphC 基因水平的增加有关。结果表明，蚯蚓通过增强生物增强柱中 PCB 降解细菌的分散以及提供有利于本地 PCB 降解细菌生长和活性的环境条件来促进 PCB 生物修复。 (C) 2002 年由 Elsevier Science B.V. 代表欧洲微生物学会联合会出版。
Partial bioremediation of polychlorinated biphenyl (PCB)-contaminated soils has been achieved using bioaugmentation with PCB-degrading bacteria and earthworms. To further study the contribution of earthworms to bioremediation, an experiment was conducted in which the changes in indigenous and bioaugmented PCB-degrading bacteria were analyzed during treatment of contaminated soil using earthworms (Pheretima hawayana) alone or in combination with the PCB-degrading bacteria, Ralstonia eutrophus and Rhodococcus sp. ACS. Bacteria used for bioaugmentation were induced with carvone and salicylic acid in culture and were repeatedly applied every 3-4 days to the surface of unmixed, 20-cm long soil columns containing 100 ppm Aroclor 1242. After 9 weeks of treatment, the soil bacterial communities were analyzed using PCR primers for the bph genes. Results showed that approximately 50% of the PCBs were removed in the top 9 cm using a combination of earthworms and bioaugmentation, whereas bioaugmentation or earthworms applied alone were effective only for removing PCBs from the top 3 cm of the soil columns. Enhanced removal of PCBs caused by earthworms was associated with an increase in the population size of culturable, indigenous biphenyl-degrading bacteria, and an increase in the level of the bphA and bphC genes. The results suggest that earthworms facilitate PCB bioremediation by enhancing the dispersal of PCB-degrading bacteria in bioaugmented columns, as well as providing environmental conditions that favor the growth and activity of indigenous PCB-degrading bacteria. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.