Overexpression of MtWRKY76 increases both salt and drought tolerance in Medicago truncatula
Overexpression of MtWRKY76 increases both salt and drought tolerance in Medicago truncatula
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MtWRKY76 的过表达提高了蒺藜苜蓿的耐盐性和耐旱性
DOI：
10.1016/j.envexpbot.2015.10.007
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发表时间：
2016-03-01
影响因子：
5.7
通讯作者：
Wang, Tao
中科院分区：
文献类型：
--
作者：
Liu, Liping;Zhang, Zhenqian;Wang, Tao
WRKY 转录因子已在多种植物物种中进行了研究，但在豆科植物中仍然很大程度上未被表征。为了深入了解WRKY在豆科植物中的功能，我们从蒺藜苜蓿中分离并鉴定了WRKY家族的一个成员MtWRKY76，其属于亚组II。表达分析表明MtWRKY76被非生物胁迫快速诱导。 MtWRKY76 过表达显着增强了转基因蒺藜藻的耐盐性和耐旱性，并触发了非生物诱导基因。 MtWRKY76 位于细胞核中，与 Medtr1g098680.1 相互作用，Medtr1g098680.1 被推导为 ASR 蛋白，该蛋白是参与非生物胁迫信号通路的转录调节复合物的组成部分。有趣的是，通过 Solexa/Illumina 数字基因表达 (DGE) 分析发现 MtWRKY76 参与非生物调控途径。 总之，我们证明 MtVVRICY76 是蒺藜藻中的多功能非生物胁迫反应基因。 (C) 2015 Elsevier B.V. 保留所有权利。
WRKY transcription factors have been studied in a wide range of plant species, but have remained largely uncharacterized in legumes. To obtain insight into the functions of WRKY in legumes, we isolated and characterized one WRKY family member from Medicago truncatula,MtWRKY76, which belongs to subgroup II.Expression analysis showed that MtWRKY76 was rapidly induced by abiotic stresses. MtWRKY76 overexpression markedly enhanced the salt and drought tolerance of transgenic M. truncatula and triggered abiotic inducible genes. MtWRKY76 was located in the nucleus and interacted with Medtr1g098680.1 that was deduced as ASR protein, which acted as a component of a transcription regulating complex involved in abiotic stress signalling pathways. Interestingly, MtWRKY76 was found to be involved in regulated abiotic pathways via Solexa/Illumina digital gene expression (DGE) analysis.In conclusion, we demonstrated that MtVVRICY76 was a multifunctional abiotic stress response gene in M. truncatula. (C) 2015 Elsevier B.V. All rights reserved.