Metabolomic differential display analysis of the white-rot basidiomycete Phanerochaete chrysosporium grown under air and 100% oxygen.
Metabolomic differential display analysis of the white-rot basidiomycete Phanerochaete chrysosporium grown under air and 100% oxygen.
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DOI：
10.1111/j.1574-6968.2004.tb09521.x
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发表时间：
2004-05
影响因子：
2.1
通讯作者：
D. Miura;Hiroo Tanaka;H. Wariishi
中科院分区：
文献类型：
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作者：
D. Miura;Hiroo Tanaka;H. Wariishi
当白腐担子菌黄孢原毛革菌的培养气氛从空气变为100%氧气时，在2-3天内观察到冻干菌丝体重量增加，胞外葡聚糖层增厚。为了更好地理解黄孢原毛平革菌对氧胁迫的响应机制，采用代谢组学差异显示技术对空气和100%O（2）气氛下培养的真菌细胞代谢产物进行了分析。在真菌细胞甲醇提取物的GC-MS总离子色谱图中，至少检测到183个峰，鉴定出53种化合物。其中，藜芦醇（VA），苏糖酸盐，和藜芦膦酸盐被确定为氧应激反应的代谢产物。在氧胁迫后1 h内，细胞内VA浓度急剧增加，表明黄孢原毛平革菌VA的产生对氧胁迫敏感。
When the cultural atmosphere of the white-rot basidiomycete, Phanerochaete chrysosporium, was changed from air to 100% oxygen, the lyophilized mycelial weight increased and thickening of extracellular glucan layer was observed in 2-3 days. To better understand the oxygen-stress responsive mechanism of P. chrysosporium, the metabolomic differential display analysis was performed using metabolites isolated from fungal cells grown under either air or 100% O(2) atmosphere. In the GC-MS total ion chromatogram of methanol-extracts from fungal cells, at least 183 peaks were detected and 53 compounds were identified. Among them, veratryl alcohol (VA), threonate, and erythronate were identified as oxygen-stress responsive metabolites. The intracellular concentration of VA increased dramatically within 1 h after an oxygen purge, indicating that VA production is sensitive to the oxygen stress in P. chrysosporium.