Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system
Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system
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无细胞系统对 ComXRO-E-2 信息素色氨酸残基的香叶基修饰
DOI：
10.1016/j.febslet.2011.12.012
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发表时间：
2012
期刊：
影响因子：
3.5
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Tsuji;F.;Ishihara;A.;Kurata;K.;Nakagawa;A.;Okada;M.;Kitamura;S.;Kanamaru;K.;Masuda;Y.;Murakami;K.;Irie;K.;Sakagami;Y.
ComX信息素是含有修饰的色氨酸残基的类异戊二烯寡肽，其刺激革兰氏阳性细菌芽孢杆菌中的天然遗传能力。由于色氨酸残基上的翻译后异戊烯化除了在ComX信息素中还没有报道，这种修饰的普遍性尚未得到阐明。在本文中，我们建立了一个无细胞的系统，从而在肽中的色氨酸残基的修饰与香叶基修饰酶ComQ。此外，我们研究了酶的反应条件，使用在体外酶反应系统。这是首次报道色氨酸残基的体外香叶基化。该系统是一个潜在的有用的工具，用于阐明的普适性上的色氨酸残基的异戊二烯化。
ComX pheromone is an isoprenoidal oligopeptide containing a modified tryptophan residue, which stimulates natural genetic competence in the gram-positive bacterium Bacillus. Since posttranslational prenylation on the tryptophan residue has not been reported except in ComX pheromone, the universality of this modification has not yet been elucidated. In this paper, we established a cell-free system, whereby the tryptophan residue in peptides is modified with a geranyl group by modifying enzyme ComQ. In addition, we investigated enzymatic reaction conditions using an in vitro enzyme reaction system. This is the first report of in vitro geranylation on the tryptophan residue. This system is potentially a useful tool for elucidating the universality of prenylation on the tryptophan residue.