Suppression of mutations in mitochondrial DNA by tRNAs imported from the cytoplasm
Suppression of mutations in mitochondrial DNA by tRNAs imported from the cytoplasm
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DOI：
10.1126/science.289.5486.1931
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发表时间：
2000-09-15
期刊：
影响因子：
56.9
通讯作者：
Tarassov, IA
中科院分区：
文献类型：
--
作者：
Kolesnikova, OA;Entelis, NS;Tarassov, IA
酵母细胞质转移RNA （tRNA）的线粒体输入需要蛋白前体输入机制和细胞质因子。我们研究了tRNA输入通路是否可以用于纠正线粒体DNA突变导致的呼吸器缺陷，以及该系统是否可以转移到人体细胞中。我们发现，具有改变的氨基酰化特性的细胞质trna可以特异性地靶向线粒体并参与线粒体翻译。我们还表明，通常不进口tRNA的人类线粒体能够在体外内化酵母tRNA衍生物，并且这种进口需要酵母进口因子。
Mitochondrial import of a cytoplasmic transfer RNA (tRNA) in yeast requires the preprotein import machinery and cytosolic factors. We investigated whether the tRNA import pathway can be used to correct respirator,, deficiencies due to mutations in the mitochondrial DNA and whether this system can be transferred into human cells. We show that cytoplasmic tRNAs with altered aminoacylation identity can be specifically targeted to the mitochondria and participate in mitochondrial translation. We also show that human mitochondria, which do not normally import tRNAs, are able to internalize yeast tRNA derivatives in vitro and that this import requires an essential yeast import factor.