microRNA expression profiling of endometrial endometrioid adenocarcinomas and serous adenocarcinomas reveals profiles containing shared, unique and differentiating groups of microRNAs.
microRNA expression profiling of endometrial endometrioid adenocarcinomas and serous adenocarcinomas reveals profiles containing shared, unique and differentiating groups of microRNAs.
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DOI：
10.3892/or.2011.1372
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发表时间：
2011-10
期刊：
影响因子：
4.2
通讯作者：
Leslie KK
中科院分区：
文献类型：
--
作者：
Devor EJ;Hovey AM;Goodheart MJ;Ramachandran S;Leslie KK
microRNA（miRNAs）控制人类癌症中的多种途径。同一类型组织中不同组织学类型肿瘤之间的miRNA差异表达提供了对发病机制的深入了解，并可能有助于指导治疗以改善预后。我们使用从良性子宫内膜和原发性子宫内膜肿瘤中提取的RNA评估了667种miRNAs在子宫内膜类腺瘤和浆液性腺癌中的表达。子宫内膜腺癌的定量miRNA分析显示四组重叠的显著过表达和低表达的miRNA。第一组由与良性子宫内膜相比在两种腺癌类型中显著失调的20个miRNA组成，两组由与良性子宫内膜相比在类腺瘤样腺癌或浆液性腺癌中显著失调的miRNA组成，第四组由17个显著区分类腺瘤样腺癌和浆液性腺癌本身的miRNA组成。在由10个类胶质瘤和5个浆液性肿瘤组成的第二组中进行所选miRNA表达水平的验证。使用已发表的来源鉴定这些失调的miRNA的实验验证的mRNA靶，而TargetScan用于预测第一和第四概况组中的miRNA的靶。这些经验证的和潜在的miRNA靶列表使用与良性子宫内膜相比在子宫内膜癌中显示显著过表达或低表达的基因的公开列表进行筛选。我们的研究结果揭示了一些在子宫内膜癌（和其他）中常见的失调miRNAs，以及一些以前在子宫内膜癌中未发现的失调miRNAs。了解这些差异可能有助于发展预后和诊断生物标志物。
microRNAs (miRNAs) control a multitude of pathways in human cancers. Differential expression of miRNAs among different histological types of tumors within the same type of tissue offers insight into the mechanism of pathogenesis and may help to direct treatment to improve prognosis. We assessed expression of 667 miRNAs in endometrial endometrioid and serous adenocarcinomas using RNA extracted from benign endometrium as well as from primary endometrial tumors. Quantitative miRNA profiling of endometrial adenocarcinomas revealed four overlapping groups of significantly overexpressed and underexpressed miRNAs. The first group was composed of 20 miRNAs significantly dysregulated in both adenocarcinoma types compared with benign endometrium, two groups were composed of miRNAs significantly dysregulated in either endometrioid adenocarcinomas or in serous adenocarcinomas compared with benign endometrium, and the fourth group was composed of 17 miRNAs that significantly distinguished between endometrioid adenocarcinomas and serous adenocarcinomas themselves. Validation of the expression levels of the selected miRNAs was carried out in a second panel composed of ten endometrioid and five serous tumors. Experimentally validated mRNA targets of these dysregulated miRNAs were identified using published sources, whereas TargetScan was used to predict targets of miRNAs in the first and fourth profile groups. These validated and potential miRNA target lists were filtered using published lists of genes displaying significant overexpression or underexpression in endometrial cancers compared to benign endometrium. Our results revealed a number of dysregulated miRNAs that are commonly found in endometrial (and other) cancers as well as several dysregulated miRNAs not previously identified in endometrial cancers. Understanding these differences may permit the development of both prognostic and diagnostic biomarkers.
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影响因子：
11.2
作者：
Huang YW;Liu JC;Deatherage DE;Luo J;Mutch DG;Goodfellow PJ;Miller DS;Huang TH
通讯作者：
Huang TH
影响因子：
4.7
作者：
Boren, Todd;Xiong, Yin;Lancaster, Johnathan M.
通讯作者：
Lancaster, Johnathan M.
影响因子：
5.7
作者：
Hiroki, Eri;Akahira, Jun-ichi;Yaegashi, Nobuo
通讯作者：
Yaegashi, Nobuo
影响因子：
11.2
作者：
Byun, Hyang-Min;Wong, Hui-Lee;Yang, Allen S.
通讯作者：
Yang, Allen S.
影响因子：
8
作者：
Dugimont, T;Montpellier, C;Curgy, JJ
通讯作者：
Curgy, JJ