Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer.
Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer.
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DOI：
10.1186/1471-2407-12-624
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发表时间：
2012-12-28
期刊：
影响因子：
3.8
通讯作者：
Klinge CM
中科院分区：
文献类型：
--
作者：
Jala VR;Radde BN;Haribabu B;Klinge CM
据报道，G蛋白偶联的雌激素受体（GPER/GPR30）结合17β-雌二醇（E2），他莫昔芬和ICI 182,780（Fulvestorant），并促进表皮生长因子受体（EGFR）介导的激活，甲状腺癌细胞。研究是检查GPER在肺癌中的表达。
使用标准的定量实时PCR（在mRNA水平），蛋白质印迹和免疫组织化学（IHC）方法（在蛋白质水平上），对各种肺癌线和肺部肿瘤的表达模式进行了研究。进行了比较（癌症与邻近组织以及癌症与正常肺组织）。
实时PCR和Western印迹分析显示，与永生的正常肺支气管上皮细胞（HBEC）相对于人类非小细胞肺癌细胞（NSCLC）线（NSCLC）线的GPE表达明显更高。小气道上皮细胞系HPL1D比HBEC表现更高，与NSCLC细胞的表达相似。鼠肺腺瘤以及人肺腺癌，鳞状细胞癌和非小细胞肺癌的切片始终显示出GPER在肿瘤中相对于周围非肿瘤组织的较高表达。
这项研究的结果表明，与正常肺部相比，肺癌细胞和肿瘤的GPEr表达增加了。
G-protein-coupled estrogen receptor (GPER/GPR30) was reported to bind 17β-estradiol (E2), tamoxifen, and ICI 182,780 (fulvestrant) and promotes activation of epidermal growth factor receptor (EGFR)-mediated signaling in breast, endometrial and thyroid cancer cells. Although lung adenocarcinomas express estrogen receptors α and β (ERα and ERβ), the expression of GPER in lung cancer has not been investigated. The purpose of this study was to examine the expression of GPER in lung cancer. The expression patterns of GPER in various lung cancer lines and lung tumors were investigated using standard quantitative real time PCR (at mRNA levels), Western blot and immunohistochemistry (IHC) methods (at protein levels). The expression of GPER was scored and the pairwise comparisons (cancer vs adjacent tissues as well as cancer vs normal lung tissues) were performed. Analysis by real-time PCR and Western blotting revealed a significantly higher expression of GPER at both mRNA and protein levels in human non small cell lung cancer cell (NSCLC) lines relative to immortalized normal lung bronchial epithelial cells (HBECs). The virally immortalized human small airway epithelial cell line HPL1D showed higher expression than HBECs and similar expression to NSCLC cells. Immunohistochemical analysis of tissue sections of murine lung adenomas as well as human lung adenocarcinomas, squamous cell carcinomas and non-small cell lung carcinomas showed consistently higher expression of GPER in the tumor relative to the surrounding non-tumor tissue. The results from this study demonstrate increased GPER expression in lung cancer cells and tumors compared to normal lung. Further evaluation of the function and regulation of GPER will be necessary to determine if GPER is a marker of lung cancer progression.
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影响因子：
3.8
作者：
Arias-Pulido, Hugo;Royce, Melanie;Gong, Yun;Joste, Nancy;Lomo, Lesley;Lee, Sang-Joon;Chaher, Nabila;Verschraegen, Claire;Lara, Juanita;Prossnitz, Eric R.;Cristofanilli, Massimo
通讯作者：
Cristofanilli, Massimo
影响因子：
11.2
作者：
Ariazi EA;Brailoiu E;Yerrum S;Shupp HA;Slifker MJ;Cunliffe HE;Black MA;Donato AL;Arterburn JB;Oprea TI;Prossnitz ER;Dun NJ;Jordan VC
通讯作者：
Jordan VC
影响因子：
51.1
作者：
Dubey, Sarita;Siegfried, Jill M.;Traynor, Anne M.
通讯作者：
Traynor, Anne M.
影响因子：
4.1
作者：
Ivanova MM;Mazhawidza W;Dougherty SM;Minna JD;Klinge CM
通讯作者：
Klinge CM
DOI：
10.1073/pnas.91.18.8517
发表时间：
1994-08-30
影响因子：
11.1
作者：
ARONICA, SM;KRAUS, WL;KATZENELLENBOGEN, BS
通讯作者：
KATZENELLENBOGEN, BS