Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis
Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis
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DOI：
10.1016/j.tripleo.2009.10.006
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发表时间：
2010-03-01
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影响因子：
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通讯作者：
Bagnato, Vanderlei Salvador
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文献类型：
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作者：
de Oliveira Mima, Ewerton Garcia;Pavarina, Ana Claudia;Bagnato, Vanderlei Salvador
目标。口腔念珠菌病动物模型中抗菌PDT的体内研究很少，卟啉和LED光的关联尚未被评估为念珠菌的体内光失活。本研究评价了光动力疗法（PDT）对体内白色念珠菌灭活的效果。研究设计。71只6周龄瑞士雌性小鼠免疫抑制，在饮水中加入四环素，然后口服白色念珠菌悬液（107 CFU/mL）。口服接种4天后，局部给药400mg /L、500mg /L或1000mg /L的photom后，在舌背进行PDT， 30分钟后用LED灯（305j /cm(2)）在455或630 nm照射（每个n = 5）。从舌部拭取酵母后，测定存活酵母细胞数（CFU/mL），并采用方差分析和Holm-Sidak检验进行分析（P < 0.05）。动物被人道地杀死，舌头被手术切除并处理，以进行酵母和炎症反应的组织学评估。与阳性对照组相比，PDT导致舌部白色念珠菌数量显著减少（P < 0.001）。使用的phogome和LED光波长的浓度之间没有差异。舌的组织学评价显示，PDT对局部粘膜没有明显的不良影响。PDT可显著降低白色念珠菌生物膜的活力，但不损害舌组织。（口腔外科口腔医学口腔病理口腔放射医学杂志2010；10:392-401）
Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated.Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (107 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm(2)) at 455 or 630 nm (n = 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P < .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction.Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P < .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa.Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: 392-401)