Comparison of α7 nicotinic acetylcholine receptor development in the hippocampal formation of C3H and DBA/2 mice
Comparison of α7 nicotinic acetylcholine receptor development in the hippocampal formation of C3H and DBA/2 mice
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DOI：
10.1016/s0165-3806(03)00106-8
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发表时间：
2003-07-12
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影响因子：
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通讯作者：
Adams, CE
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作者：
Adams, CE
据报道，α 7受体基因位点的菌株特异性限制性内切片段长度多态性显著影响成年小鼠海马中烟碱受体α 7亚型的表达。本研究采用α -班加罗毒素（α - btx）放射自显影技术，对具有不同α - 7受体基因位点等位基因的两种小鼠品系（C3H和DBA/2）海马区α - 7受体的发育进行了研究。在胚胎第13天（E13），在胚胎C3H小鼠的海马背侧区首次检测到α - btx的结合。相比之下，在E16的DBA/2菌株中，最初主要在海马区CA3检测到α - btx结合。在这两个菌株的神经上皮中都没有α - btx的结合。新生儿C3H小鼠海马CA1区α - btx结合明显增加，CA3区在E18至出生后第5天（P5）之间有较小程度的增加，而DBA/2小鼠没有观察到这种增加。在出生后第一个月结束时，海马α - btx结合在每个品系中都表现出成人样。这些数据表明，alpha7受体基因位点的变化对小鼠海马中alpha7受体的发育表达有不同的影响。因此，在C3H和DBA/2小鼠中，由于alpha7受体表达的时间过程不同，alpha7受体对细胞迁移、树突细化和/或轴突连接等发育过程的潜在影响可能表现出菌株选择性差异。(C) 2003 Elsevier B.V.版权所有
A strain-specific restriction fragment length polymorphism in the alpha7 receptor gene locus has been reported to significantly affect the expression of the alpha7 subtype of nicotinic receptor in adult mouse hippocampus. The goal of the present study was to characterize the development of the alpha7 receptor in hippocampus from two mouse strains (C3H and DBA/2) with different alleles of the alpha7 receptor gene locus by using alpha-bungarotoxin (alpha-BTX) autoradiography. Binding of alpha-BTX was initially detected in fetal C3H mice on embryonic day 13 (E13) in the dorsal portion of the hippocampal anlage. In contrast, alpha-BTX binding was initially detected primarily in hippocampal area CA3 in the DBA/2 strain on E16. Binding of alpha-BTX was absent from the neuroepithelium in both strains. A marked increase in alpha-BTX binding was observed in hippocampal area CA1 and to a lesser extent in area CA3 between E18 and postnatal day 5 (P5) in neonatal C3H mice, an increase that was not observed in the DBA/2 mice. By the end of the first postnatal month, hippocampal alpha-BTX binding appeared adult-like in each strain. These data suggest that variations in the alpha7 receptor gene locus differentially influence the developmental expression of the alpha7 receptor in murine hippocampus. Therefore, the potential influence of the alpha7 receptor on developmental processes such as cell migration, dendritic elaboration and/or axonal connectivity may exhibit strain-selective differences because of the dissimilar time courses of alpha7 receptor expression in C3H and DBA/2 mice. (C) 2003 Elsevier B.V. All rights reserved.