The utility of DLA typing for transplantation medicine in canine models
The utility of DLA typing for transplantation medicine in canine models
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DOI：
10.1292/jvms.20-0142
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发表时间：
2020-08-01
影响因子：
1.2
通讯作者：
Moritomo, Tadaaki
中科院分区：
文献类型：
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作者：
Sato, Keita;Miyamae, Jiro;Moritomo, Tadaaki
移植医学用于治疗严重的犬疾病，犬白细胞抗原（DLA）被认为是移植排斥反应的重要因素。然而，DLA I类和II类的直接测序的效用尚未得到彻底评估。将8只具有DLA基因的健康比格犬分为两组，每组4只狗，每组包括1只供体和3只受体用于皮肤移植。选择以下受体：一只完全匹配的狗，一只具有单倍性匹配，一只与供体的DLA基因完全不匹配。从每个供体获得全层皮肤片段并移植到受体。进行混合淋巴细胞反应（MLR）试验，并通过流式细胞术进行分析。DLA半相合和不匹配的皮肤移植物在14天内被严重排斥，而在完全匹配的DLA对中，存活期长达21天。组织学评价也显示中度至重度淋巴细胞浸润和坏死的DLA不匹配对。在MLR试验中，两组中DLA不匹配对的刺激指数均显著高于完全匹配的DLA对（P
Transplantation medicine is used for the treatment of severe canine diseases, and the dog leukocyte antigen (DLA) is considered to be important in graft rejection. However, the utility of direct sequencing of both DLA classes I and II has not been assessed thoroughly. Eight healthy beagles with identified DLA genes were divided into two sets of four dogs, each including one donor and three recipients for skin transplantation. The following recipients were selected: one dog with a complete match, one with a haploidentical match, and one with a complete mismatch of the DLA gene with the donor. Full-thickness skin segments were obtained from each donor and transplanted to the recipients. A mixed lymphocyte reaction ( MLR) assay was performed and analyzed by flow cytometry. Skin grafts of DLA haploidentical and mismatched pairs were grossly rejected within 14 days, whereas in fully matched DLA pairs, survival was as long as 21 days. Histopathological evaluation also showed moderate to severe lymphocytic infiltration and necrosis in DLA mismatched pairs. As seen in the MLR assay, the stimulation index of DLA mismatched pairs was significantly higher than that of fully matched DLA pairs in both sets (P