Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.
Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.
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DOI：
10.2147/ijn.s77816
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发表时间：
2015
影响因子：
8
通讯作者：
Amler E
中科院分区：
文献类型：
--
作者：
Plencner M;Prosecká E;Rampichová M;East B;Buzgo M;Vysloužilová L;Hoch J;Amler E
切口疝气是最常见的术后并发症，影响高达20%的腹部手术后患者。人工合成的外科网片已经成为腹股沟修补术的标准护理。然而，网状物的实施并不能降低复发的风险，并且腹股沟复发的开始只推迟了2-3年。如今，市场上有100多种外科网片可供选择，其中聚丙烯是最广泛用于腹股沟修补的网片。尽管如此，理想的网格还不存在；它仍然需要开发。聚己内酯纳米纤维似乎是一种适合不同类型的细胞的材料，包括成纤维细胞、软骨细胞和间充质干细胞。本研究的目的是开发一种用于腹股沟再生的功能化支架。我们制备了一种新型的复合支架，该支架由聚ε-己内酯(PCL)纳米纤维功能化的聚丙烯外科网片和作为生长因子天然来源的黏附的血小板组成。在广泛的体外测试中，我们证明了PCL纳米纤维与沉积在聚丙烯网状物上的黏附的血小板具有生物相容性。与单独的聚丙烯网状物相比，这种复合支架在所有测试中都能提供更好的小鼠成纤维细胞的粘附性、生长、代谢活性、增殖和活力，甚至优于由PCL纳米纤维功能化的聚丙烯网状物。从生物相容的纳米纤维修饰支架中逐渐释放生长因子似乎是组织工程和再生医学中一种很有前途的方法。
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2–3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.