A fluorescent probe for Hg2+ sensing in solutions and living cells with a wide working pH range
A fluorescent probe for Hg2+ sensing in solutions and living cells with a wide working pH range
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DOI：
10.1016/j.cclet.2013.10.027
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发表时间：
2014
影响因子：
9.1
通讯作者：
Zhixiang Han;Bo-Song Zhu;Tong-Liang Wu;Qingqing Yang;Yong-Lai Xue;Zhen Zhang;Xiangyang Wu
中科院分区：
文献类型：
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作者：
Zhixiang Han;Bo-Song Zhu;Tong-Liang Wu;Qingqing Yang;Yong-Lai Xue;Zhen Zhang;Xiangyang Wu
本文合成了2-羧基苯甲醛罗丹明B硫代肼（1），并在DMF/H_2O（1：9，v/v）溶液中作为荧光探针对Hg ~（2+）进行了高选择性识别。该探针可用于汞离子的定量测定，线性范围为1.0 × 10− 7 ~ 1.0 × 10− 5 mol/L（R2= 0.9985），检出限为4.2 × 10− 8 mol/L。实验结果表明，探针1对Hg ~（2+）的响应在4.0 ~ 9.0的范围内与pH无关。此外，探针1对Hg 2+的选择性优于其他常见的金属阳离子。最重要的是，该探针可用于活细胞中Hg ~（2+）的荧光成像监测，获得了满意的结果。
In this paper, 2-carboxybenzaldehyde rhodamine B thiohydrazine (1) was synthesized and developed as a fluorescent probe to recognize Hg2+in DMF/H2O (1:9, v/v) solution with high selectivity. The probe can be applied to the quantification of Hg2+with a linear concentration range covering from 1.0 × 10−7mol/L to 1.0 × 10−5mol/L (R2= 0.9985) and a detection limit of 4.2 × 10−8mol/L. The experiment results show that the response of probe1to Hg2+is pH-independent in a wide range from 4.0 to 9.0. Moreover, the probe1exhibits excellent selectivity toward Hg2+over other common metal cations. Most importantly, the probe can be employed to monitor Hg2+in living cells using fluorescent imaging technique with satisfied results.