Direct electron transfer of PQQ-glucose dehydrogenase at modified carbon nanotubes electrodes
Direct electron transfer of PQQ-glucose dehydrogenase at modified carbon nanotubes electrodes
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DOI：
10.1016/j.elecom.2011.08.034
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发表时间：
2011-11-01
影响因子：
5.4
通讯作者：
Lisdat, F.
中科院分区：
文献类型：
--
作者：
Goebel, G.;Schubart, I. W.;Lisdat, F.
为了建立有效的酶电极接触的吡咯喹啉醌依赖性葡萄糖脱氢酶（PQQ-GDH）不同的固定化策略进行了研究。通过化学处理和聚苯胺衍生物修饰多壁碳纳米管（MWCNT）的金电极。甲氧基-间-苯胺磺酸和间-氨基苯甲酸在MWCNT上的电聚合允许PQQ-GDH的共价偶联。这种聚-[ASA-ABA]/MWCNT/Au电极在相对于Ag/AgCl为100 mV的电位下可以实现高达500 μ A/cm（2）的电流密度。此外，少量游离PQQ的研究表明酶和电极之间的直接电子转移。(C)2011 Elsevier B. V.保留所有权利。
In order to establish efficient enzyme-electrode-contacts for the pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) different immobilisation strategies are investigated. Multi-walled carbon nanotubes (MWCNT) on gold electrodes are modified by chemical treatment and by (poly)-aniline derivatives. The electropolymerisation of methoxy-m-anilinesulfonic acid and m-aminobenzoic acid on the MWCNTs allows the covalent coupling of the PQQ-GDH. Such a poly-[ASA-ABA]/MWCNT/Au electrode can achieve current densities of up to 500 mu A/cm(2) at a potential of 100 mV vs. Ag/AgCl. Furthermore investigations with small amounts of free PQQ indicate direct electron transfer between enzyme and electrode. (C) 2011 Elsevier B.V. All rights reserved.