Inkjet-printed microfluidic multianalyte chemical sensing paper
Inkjet-printed microfluidic multianalyte chemical sensing paper
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DOI：
10.1021/ac800604v
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发表时间：
2008-09-15
影响因子：
7.4
通讯作者：
Citterio, Daniel
中科院分区：
文献类型：
--
作者：
Abe, Koji;Suzuki, Koji;Citterio, Daniel
本文提出了一种用于制造整个由适合定量分析的纸制成的整个微流体化学传感设备的喷墨打印方法，仅需要单个打印设备。喷墨打印设备用于制造三维亲水性微流体图案（550 mu m宽流通通道）和感应区域（1.5 mm x 1.5 mm x 1.5 mm squares）在滤纸上，通过喷墨蚀刻，从而局部溶解一个疏水聚（苯乙烯）层通过将滤纸浸泡在甲苯中的聚（苯乙烯）1 wt％的溶液中获得。在第二步中，相同的喷墨打印设备用于打印“化学传感墨水”，其中包含比色分析测定的必要试剂，以进入定义明确的微流体纸设备的明确区域。优化了图案，印刷油墨和感应区域的布置以获得均匀的颜色响应。结果是用于临床相关浓度范围的pH，总蛋白和葡萄糖的同时测定尿液分析（人血清白蛋白的0.46-46 mu，葡萄糖2.8-28.0 mm）的“全墨射”化学感应器和pH 5-9）。定量数据是通过颜色扫描仪和简单的计算机程序在L*a*b*颜色空间中通过数字颜色分析获得的。
This paper presents an inkjet printing method for the fabrication of entire microfluidic multianalyte chemical sensing devices made from paper suitable for quantitative analysis, requiring only a single printing apparatus. An inkjet printing device is used for the fabrication of three-dimensional hydrophilic microfluidic patterns (550-mu m-wide flow channels) and sensing areas (1.5 mm x 1.5 mm squares) on filter paper, by inkjet etching, and thereby locally dissolving a hydrophobic poly(styrene) layer obtained by soaking of the filter paper in a 1 wt % solution of poly(styrene) in toluene. In a second step, the same inkjet printing device is used to print "chemical sensing inks", comprising the necessary reagents for colorimetric analytical assays, into well-defined areas of the patterned microfluidic paper devices. The arrangement of the patterns, printed inks, and sensing areas was optimized to obtain homogeneous color responses. The results are "all-inkjet-printed" chemical sensing devices for the simultaneous determination of pH, total protein, and glucose in clinically relevant concentration ranges for urine analysis (0.46-46 mu M for human serum albumin, 2.8-28.0 mM for glucose, and pH 5-9). Quantitative data are obtained by digital color analysis in the L*a*b* color space by means of a color scanner and a simple computer program.