Histochemical study on heterogeneity of lignin in Eucalyptus species II.: The distribution of lignins and polyphenols in the walls of various cell types
Histochemical study on heterogeneity of lignin in Eucalyptus species II.: The distribution of lignins and polyphenols in the walls of various cell types
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DOI：
10.1163/22941932-90000366
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发表时间：
2004-01-01
期刊：
影响因子：
1.9
通讯作者：
Funada, R
中科院分区：
文献类型：
--
作者：
Watanabe, Y;Kojima, Y;Funada, R
采用Maule显色反应结合显微分光光度法和紫外显微分光光度法，研究了多酚类物质对赤桉（Eucalyptus camldulensis）和赤桉（Eucalyptus）木材木质素分析的影响。球状体。用不同浓度的碱溶液提取薄片和木粉。碱溶性浸出物的量随着NaOH浓度的增加而增加。相比之下，Klason木质素的量与NaOH的浓度之间没有明显的相关性。碱抽提后，两种木本植物细胞壁的可见光吸收光谱发生了变化。特别是，细胞壁的容器元素的光谱变化很大，即使只有稀溶液的碱。紫外吸收光谱经碱提取后无明显变化。这些结果表明，细胞壁中的多酚影响组织化学分析的结果。因此，需要用碱进行初步提取，即用1%的NaOH溶液进行提取，以通过组织化学方法评估桉树木材细胞壁中木质素的精确分布。赤桉木材纤维细胞壁中含有愈创木基和异戊烯基，导管壁中主要含有愈创木基。蓝桉木材纤维细胞壁主要含有愈创木基单元，导管分子细胞壁同时含有愈创木基和愈创木基单元。丁香基型多酚，其中具有光谱相似的丁香基型木质素，被发现在木材纤维和导管元件的细胞壁和木材纤维之间的细胞角在这两个物种的桉树。
We examined the effects of polyphenols on the analysis of lignin by histochemical methods, namely, the Maule color reaction coupled with microspectrophotometry and ultraviolet micro spec trophotometry, in wood of Eucalyptus camldulensis and E. globulus. Thin sections and wood meals were extracted with solutions of alkali at different concentrations. The amounts of alkali-soluble extractives increased with increasing concentrations of NaOH. By contrast, there was no clear correlation between amounts of Klason lignin and the concentration of NaOH. The visible-light absorption spectra of cell walls of all woody tissues from both species changed after alkali extraction. In particular, the spectra of cell walls of vessel elements changed considerably, even when only a dilute solution of alkali was used. Ultraviolet absorption spectra did not show clear changes after extraction with alkali. These results indicate that polyphenols in cell walls affect the results of histochemical analysis. Therefore, a preliminary extraction with alkali, namely, extraction with a 1% solution of NaOH, is needed to assess the precise distribution of lignins in the cell walls of Eucalyptus wood by histochemical methods. The cell walls of wood fibers of Eucalyptus camaldulensis contained both guaiacyl and syringyl units and those of vessel walls contained mostly guaiacyl units. However, the cell walls of wood fibers in Eucalyptus globulus contained mainly syringyl units, while those of vessel elements contained both guaiacyl and syringyl units. Syringyl-type polyphenols, which have spectra similar to those of syringyl-type lignins, were found in the cell walls of wood fibers and vessel elements and in cell corners among wood fibers in both species of Eucalyptus.