High-affinity [3H]inositol uptake by dissociated brain cells and cultured fibroblasts from fetal mice.
High-affinity [3H]inositol uptake by dissociated brain cells and cultured fibroblasts from fetal mice.
复制标题
胎鼠离解脑细胞和培养成纤维细胞的高亲和力[3H]肌醇摄取。
DOI：
10.1007/bf00965541
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发表时间：
1991
影响因子：
4.4
通讯作者：
Lester,BR
中科院分区：
文献类型：
--
作者：
Fruen,BR;Lester,BR
The accumulation of [3H]inositol by mechanically dissociated brain cells and cultured skin fibroblasts from fetal mice was examined. Uptake by both tissues was strongly dependent on temperature and the presence of sodium ions. Brain and fibroblast uptake also responded similarly to inhibition by inositol isomers and phloridzin. At lower concentrations of inositol, both tissues exhibited high-affinity uptake kinetics with apparent Kmvalues near 30 μM, similar to values observed previously in human fibroblasts and other cultured cells. The activity of brain high-affinity uptake was nearly an order of magnitude lower than that of fibroblasts, however, and was in part confounded by the presence of a low-affinity or simple diffusion system operating at inositol concentrations above 100μM. Brain preparation from adult mice also showed evidence of high-affinity, Na+dependent uptake, but its activity was significantly diminished relative to that of fetal brain preparations. Our results demonstrate that a high-affinity inositol transport system closely resembling that found in cultured cells is expressed in the developing mouse brain.
