Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking.
Enrichment process, structural prediction, isolation, in vitro cytotoxic and anti-inflammatory effects of triterpenoid saponins in Camellia japonica L. leaves water extract through UPLC-Q-TOF based mass spectrometry similarity networking.
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DOI：
10.1016/j.foodchem.2024.138360
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发表时间：
2024-01
期刊：
影响因子：
8.8
通讯作者：
Hui Liu;Lisha Bi;Qirun Chen;Xiaozhi He;Huan Yan;W. Ni;Wenjuan Wu;Li He;Haiyang Liu
中科院分区：
文献类型：
--
作者：
Hui Liu;Lisha Bi;Qirun Chen;Xiaozhi He;Huan Yan;W. Ni;Wenjuan Wu;Li He;Haiyang Liu
山茶属(Camellia Japan onicaL.)富含生物活性化合物，但其增进健康的潜力往往被其观赏价值所掩盖。值得注意的是，三萜皂苷因其表面活性而备受关注。MolNetEnhancer揭示了537种化合物公司。海参叶水提物，分为32类，其中三萜皂苷38种。采用D101树脂柱层析分离纯化三萜皂苷。基于UPLC-Q-TOF的分子网络分析和基于HPLC的定量分析表明，皂苷主要集中在组分3和组分4(68.3%转移)。MS2LDA和NAP预测了38个三萜皂苷的结构，揭示了其中近一半是潜在的新化合物。采用综合层析和波谱方法对三萜皂苷进行了分离纯化和结构解析，共得到13个化合物，其中7个为新化合物。统计分析和体外实验表明，这些三萜皂苷的细胞毒性和抗炎活性在抗癌作用中起着至关重要的作用。
Camellia japonicaL. is rich in bioactive compounds, but its health-enhancing potential is often overshadowed by its ornamental value. Notably, triterpenoid saponins are prominent due to their surfactant properties. MolNetEnhancer revealed 537 compounds inC. japonicaleaves water extract, classified into 32 categories, including 38 triterpenoid saponins. To enrich triterpenoid saponins, the process of D101 resin chromatography was employed. Molecular networking analysis based on UPLC-Q-TOF and quantitative analysis based on HPLC revealed saponins concentrated in fractions 3 and 4 (68.3% transfer). MS2LDA and NAP predicted structures for 38 triterpenoid saponins, revealing nearly half of them are potential new compounds. Comprehensive chromatographic and spectroscopic methods were used for purification and structural illustration of triterpenoid saponins, yielding 13, including 7 new compounds. Statistical analysis andin vitroassays revealed the cytotoxic and anti-inflammatory activities of these triterpenoid saponins played a crucial role in the anticancer effects.