Heavy metal accumulation by two earthworm species and its relationship to total and DTPA-extractable metals in soils
Heavy metal accumulation by two earthworm species and its relationship to total and DTPA-extractable metals in soils
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DOI：
10.1016/j.soilbio.2003.09.001
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发表时间：
2004-01-01
影响因子：
9.7
通讯作者：
Lavelle, P
中科院分区：
文献类型：
--
作者：
Dai, J;Becquer, T;Lavelle, P
比较了土壤中Zn、Cd、Pb和Cu的含量与污染土壤中这些重金属的总量和DTPA提取物含量。样本取自一个被70多年前农业工业废物污染的牧场。在沿着污染增加梯度的6个点采集了3个个体的Aporrectodea caliginosa和3个个体的蚯蚓和土壤样品。测定了蚯蚓、土壤和DTPA从土壤中提取的金属总含量。土壤重金属总含量范围为165.7 ~ 1231.7 mg Zn kg（-1）、2.7 ~ 5.2 mg Cd kg（-1）、45.8 ~ 465.5 mg Pb kg（-1）和30.0 ~ 107.5 mg Cu kg（-1）。它们与DTPA提取的金属具有高度显著的相关性。各组织中金属元素含量以A. caliginosa比L.红腹锦鸡儿体内Zn含量为556 ~ 3381 mg·kg ~（-1），Cd含量为11.6 ~ 102.9mg·kg ~（-1），Ph含量为1.9 ~ 182.8mg·kg ~（-1），Cu含量为17.9 ~ 35.9mg·kg ~（-1）。钙华石首鱼的锌含量为667.9 ~ 2645 mg kg-1，镉含量为7.7 ~ 26.3mg kg-1，铅含量为0.5 ~ 37.9mg kg-1，铜含量为16.0 ~ 37.6mg kg-1。rubellus，分别。蚯蚓体负荷与土壤重金属总量和DTPA提取态含量均呈显著正相关，但L. rubellus和Cu在A. caliginosa。由于DTPA提取物分析方法简单，其作为蚯蚓体内重金属生物富集的预测指标可能优于总金属含量。4种重金属的生物-土壤累积因子（BSAF）为Cd > Zn > Cu > Pb，平均值范围为：Cd（6.18-17.02），Zn（1.95-7.91），Cu（0.27-0.89），Pb（0.08-0.38）。镉（3.64-6.34）、锌（1.5-6.35）、铜（0.29-0.87）、铅（0.04-0.13）。红色。Ca、Fe、Mn的BSAF为Ca > Mn > Fe，平均值分别为：Ca（0.46-1.31）、Mn（0.041-0.111）、Fe（0.017-0.07）。红花乳杆菌中钙含量为0.98-2.13，锰含量为0.14-0.23，铁含量为0.019-0.048。主成分分析结果表明，两种植物对重金属的生物富集模式不同，这与它们在污染土壤中的生态作用有关。(C)2003 Elsevier Ltd.保留所有权利。
The concentrations of Zn, Cd, Ph and Cu in earthworm tissues were compared with the total and DTPA-extractable contents of these heavy metals in contaminated soils. Samples were taken from a pasture polluted by waste from a metallurgic industry over 70 y ago. Three individuals of Aporrectodea caliginosa and Lumbricus rubellus and soil samples were collected at six points along a gradient of increasing pollution. Total metal contents of earthworms, soil, and metals extracted by DTPA from the soil were measured. Total heavy metal contents of the soils ranged from 165.7 to 1231.7 mg Zn kg(-1), 2.7 to 5.2 mg Cd kg(-1), 45.8 to 465.5 mg Pb kg(-1) and 30.0 to 107.5 mg Cu kg(-1). Their correlations with metals extracted by DTPA were highly significant. Contents of the metals in earthworm tissues were higher in A. caliginosa than in L. rubellus, with values ranging from 556 to 3381 mg Zn kg(-1), 11.6 to 102.9 mg Cd kg(-1) 1.9 to 182.8 mg Ph kg(-1) and 17.9 to 35.9 mg Cu kg(-1) in A. caliginosa, and from 667.9 to 2645 mg Zn kg(-1), 7.7 to 26.3 mg Cd kg(-1), 0.5 to 37.9 mg Ph kg(-1) and 16.0 to 37.6 mg Cu kg(-1) in L. rubellus, respectively. Correlations between body loads in earthworms with either total or DTPA-extractable contents of soil metals were significant, except for Cd in L. rubellus and Cu in A. caliginosa. Considering its simple analytical procedure, DTPA-extractable fraction may be preferable to total metal content as a predictor of bio-concentrations of heavy metals in earthworms. Biota-to-Soil Accumulation Factor (BSAF) of these four metals are Cd > Zn > Cu > Pb, with range of mean values between: Cd (6.18-17.02), Zn (1.95-7.91), Cu (0.27-0.89) and Pb (0.08-0.38) in A. caliginosa, and Cd (3.64-6.34), Zn (1.5-6.35), Cu (0.29-0.87) and Ph (0.04-0.13) in L. rubellus. The BSAF of Ca, Fe and Mn are Ca > Mn > Fe, with mean values of: Ca(0.46-1.31), Mn (0.041-0.111), Fe (0.017-0.07) in A. caliginosa and Ca (0.98-2.13), Mn (0.14-0.23), Fe (0.019-0.048) in L rubellus, respectively. Results of principal component analysis showed that the two earthworm species differ in the pattern of metal bioaccumulation which is related to their ecological roles in contaminated soils. (C) 2003 Elsevier Ltd. All rights reserved.