Amplified detection of hepatitis B virus using an electrochemical DNA biosensor on a nanoporous gold platform
Amplified detection of hepatitis B virus using an electrochemical DNA biosensor on a nanoporous gold platform
复制标题
DOI：
10.1016/j.bioelechem.2017.06.006
复制
发表时间：
2017-10-01
影响因子：
5
通讯作者：
Mehrgardi, Masoud A.
中科院分区：
文献类型：
--
作者：
Ahangar, Laleh Enayati;Mehrgardi, Masoud A.
在本研究中，纳米多孔金平台被应用于电化学DNA生物传感器对乙肝病毒的放大检测。二茂铁作为氧化还原报告分子共价连接到DNA探针上，其电化学信号被记录为生物传感器的响应。对于真实样本，首先从患者的血液中提取DNA，然后进行5个循环的聚合酶链式反应(PCR)扩增。由于采用了纳米孔金电极，提高了传感器的灵敏度，因此该传感器能够以较低的聚合酶链式反应周期分辨出实际样品中的乙肝病毒基因组。通过这种策略和利用纳米孔平台和共价连接的电活性标记进行信号放大，该生物传感器可以区分有限的聚合酶链式反应周期的健康和乙肝患者。此外，大循环的聚合酶链式反应的误差可以忽略不计。突变DNA的线性范围为0.4~10nmoL，重复性可靠(RSD)为8.9%。(C)2017爱思唯尔B.V.保留所有权利。
In the present study, a nanoporous gold platform was applied for the amplified detection of Hepatitis B virus (HBV) by an electrochemical DNA biosensor. Ferrocene as a redox reporter was covalently attached to the DNA probe and its electrochemical signal was recorded as the biosensor response. For real samples, DNA was firstly extracted from blood of patients and then amplified by polymerase chain reaction (PCR) for 5 cycles. Sensitivity of this biosensor was enhanced by using nanoporous gold electrode, therefore this sensor can discriminate the genome of HBV in real sample with low PCR cycles. By this strategy and signal amplification using nanoporous platform and covalently attached electroactive label, the biosensor can distinguish between healthy and HBV patients with limited PCR cycles. Moreover, the errors of PCR with large cycles can be disregarded. A linear dynamic range of 0.4 to 10 nmol of mutant DNA was achieved, with reliable reproducibility (RSD) 8.9%. (C) 2017 Elsevier B.V. All rights reserved.