Rescue of a Dictyostelium discoideum mutant defective in cyclic nucleotide phosphodiesterase.
Rescue of a Dictyostelium discoideum mutant defective in cyclic nucleotide phosphodiesterase.
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环核苷酸磷酸二酯酶缺陷的盘基网柄菌突变体的拯救。
DOI：
10.1016/s0012-1606(89)80010-7
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发表时间：
1989
影响因子：
2.7
通讯作者：
Kessin,RH
中科院分区：
文献类型：
--
作者：
Faure,M;Podgorski,GJ;Franke,J;Kessin,RH
其中一种发育诱导的基因产物是环核苷酸磷酸二酯酶，它对Dictyosteliumamoeacrylate的趋化性至关重要。酶可以分泌或以膜结合形式存在。这种酶在突变体HPX235中缺失，因此，除非提供外源性cAMP磷酸二酯酶，否则不会聚集。我们已经将克隆的磷酸二酯酶基因的多个拷贝引入突变阿米巴并恢复聚集。当加入外源酶时不会发生的解剖学上正确的子实体的形成也通过用该基因转化而恢复。我们使用的构建体仅产生分泌型磷酸二酯酶，因此正常聚集和形态发生并不绝对需要该酶的膜结合形式。
One of the developmentally induced gene products that is essential for chemotaxis ofDictyosteliumamoebae is a cyclic nucleotide phosphodiesterase. The enzyme can be secreted or exist in a membrane bound form. This enzyme is missing in the mutant HPX235 which, as a consequence, does not aggregate unless exogenous cAMP phosphodiesterase is supplied. We have introduced multiple copies of the cloned phosphodiesterase gene into mutant amoebae and restored aggregation. The formation of anatomically correct fruiting bodies, which does not occur when exogenous enzyme is added, is also restored by transformation with the gene. The construct that we have used gives rise only to secreted phosphodiesterase and therefore the membrane bound form of the enzyme is not absolutely required for normal aggregation and morphogenesis.