IMMUNIZATION OF MICE WITH RECOMBINANT SJC26GST INDUCES A PRONOUNCED ANTI-FECUNDITY EFFECT AFTER EXPERIMENTAL-INFECTION WITH CHINESE SCHISTOSOMA-JAPONICUM
IMMUNIZATION OF MICE WITH RECOMBINANT SJC26GST INDUCES A PRONOUNCED ANTI-FECUNDITY EFFECT AFTER EXPERIMENTAL-INFECTION WITH CHINESE SCHISTOSOMA-JAPONICUM
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DOI：
10.1016/0264-410x(94)00045-o
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发表时间：
1995-04-01
期刊：
影响因子：
5.5
通讯作者：
MCMANUS, DP
中科院分区：
文献类型：
--
作者：
LIU, SX;SONG, GC;MCMANUS, DP
本文报道了日本血吸虫26 kDa谷胱甘肽-S转移酶(Sjc26GST)基因的克隆、表达、重组GST的亲和纯化及其在日本血吸虫26 kDa GST小鼠中的免疫效果。疫苗接种实验最显著的特点是免疫小鼠肝脏和脾中的卵子数量显著减少。在攻击感染后6周，免疫小鼠的抗Sjc26GST抗体水平显著高于对照组。这些结果与曼氏沙门氏菌(Sm28)28 kDa GST免疫动物的保护作用密切相关，据报道，在曼氏沙门氏菌攻击后，虫子存活率、虫子繁殖力和卵孵化能力都有所下降。在开发日本血吸虫病疫苗方面，Sjc26GST免疫可以在人或动物群体中提供两个互补的目标--暴露于感染或再感染后减少一些虫子负担，以及通过减少虫子繁殖力来减少病理变化的抗病效果，这种直接作用也影响到日本血吸虫的传播。
We report the cloning, by polymerase chain reaction (PCR), of a cDNA encoding a Schistosoma japonicum (Chinese) 26 kDa glufathione-S-tranferase (GST) (Sjc26GST), expression of the cDNA, affinity purification of the recombinant GST and its vaccine efficacy in outbred NIH mice using Freund's as adjuvant. The most striking feature of the vaccination experiments was the pronounced reduction in the number of eggs in the livers and spleens of immunized mice. A relatively low but significant level of protection in terms of reduced worm viability against challenge infection was also observed Further, the level of anti-Sjc26GST antibody in immunized mice was significantly higher than in control mice at week 6 post-challenge infection. These results closely mirror the protection conferred by immunization of animals with the 28 kDa GST of S. mansoni (Sm28) where a reduction in worm viability, worm fecundity and egg-hatching ability have been reported following challenge with S. mansoni. In terms of developing a vaccine against schistosomiasis japonica, immunization with Sjc26GST can provide two complementary goals in human or animal populations-some reduction in worm burden following exposure to infection or reinfection, and an anti-disease effect through reduction of pathology by a decrease in worm fecundity, with this direct effect also affecting the transmission of S. japonicum.