Axonal elongation through acellular nerve segments of the cat tibial nerve: importance of the near-nerve environment
Axonal elongation through acellular nerve segments of the cat tibial nerve: importance of the near-nerve environment
复制标题
DOI：
10.1016/s0006-8993(98)00160-7
复制
发表时间：
1998-05-11
期刊：
影响因子：
2.9
通讯作者：
Krarup, C
中科院分区：
文献类型：
--
作者：
Fugleholm, K;Sorensen, J;Krarup, C
周围神经再生被认为受远端神经残端结构、细胞和体液因素的影响。然而，轴突伸长不受猫胫神经挤压损伤远端20毫米脱细胞神经段（ANS）的影响，该神经段由硅胶袖带屏蔽。Fugleholm， H. Schmalbruch， C. Krarup，碾碎、切片和冷冻后早期周围神经再生的植入电极研究，神经科学。科学通报，14(1994):2659-2673]。在本研究中，轴突通过挤压损伤联合30、40、50、60和70毫米ANSs进行再生。对于30和40毫米的ANSs，神经被不透水的硅胶袖口屏蔽，其中包含电极，用于对轴突伸长进行电生理评估。病变9周后光镜检查所有神经组织学。通过屏蔽的30 mm ANS的延伸比通过有活细胞的屏蔽神经段的延伸慢。在离体的40 mm ANS中，观察到不完全的沃勒氏变性和血管缺乏，轴突伸长严重受损。在40-70 mm的非屏蔽ANSs中再生是完整的，再生纤维的数量与ANSs的长度没有关系，非隔离ANSs中的血管数量没有减少。结果表明，通过超过30毫米的离体脱细胞神经段再生依赖于来自近神经环境的细胞和体液支持。因此，近神经环境对于长ANSs的再生至关重要，并讨论了体液、细胞和血管支持的重要性。(C) 1998爱思唯尔科学有限公司
Peripheral nerve regeneration is considered to be influenced by structural, cellular and humoral factors in the distal nerve stump. Axonal elongation was, however, not affected by the presence of a 20 mm acellular nerve segment (ANS) distal to a crush lesion in a cat tibial nerve which was shielded from the environment by a silicone cuff [K. Fugleholm, H. Schmalbruch, C. Krarup, Early peripheral nerve regeneration after crushing, sectioning, and freeze studied by implanted electrodes in the cat, J. Neurosci., 14 (1994) 2659-2673]. In the present study axons were challenged to regenerate through crush lesions combined with 30-, 40-, 50-, 60- and 70-mm ANSs. For 30- and 40-mm ANSs, the nerves were shielded by impermeable silicone cuffs containing electrodes for electrophysiological evaluation of axonal elongation. All nerves were examined histologically by light microscopy 9 weeks after the lesion. The elongation through the shielded 30-mm ANS was slower than through a shielded nerve segment with viable cells. In the isolated 40-mm ANS, incomplete Wallerian degeneration and lack of blood vessels were observed, and axonal elongation was severely impaired. Regeneration across 40-70 mm non-shielded ANSs was intact and there was no relation between the number of regenerated fibers and the length of the ANS. There was no reduction in the number of blood vessels in the non-isolated ANSs. The results suggest that regeneration through an isolated acellular nerve segment exceeding 30 mm depends on cellular and humoral support from the near-nerve environment. Thus, the near-nerve environment is crucial for regeneration through long ANSs, and the importance of humoral, cellular and vascular support is discussed. (C) 1998 Elsevier Science B.V.