TaNF-YA7-5B, a gene encoding nuclear factor Y (NF-Y) subunit A in Triticum aestivum, confers plant tolerance to PEG-inducing dehydration simulating drought through modulating osmotic stress-associated physiological processes.
TaNF-YA7-5B, a gene encoding nuclear factor Y (NF-Y) subunit A in Triticum aestivum, confers plant tolerance to PEG-inducing dehydration simulating drought through modulating osmotic stress-associated physiological processes.
复制标题
DOI：
10.1016/j.plaphy.2022.07.036
复制
发表时间：
2022-08
期刊：
影响因子：
--
通讯作者：
Yingjia Zhao;Yanyang Zhang;Tianjiao Li;Chenyang Ni;Xinyang Bai;Ruize Lin;K. Xiao
中科院分区：
文献类型：
--
作者：
Yingjia Zhao;Yanyang Zhang;Tianjiao Li;Chenyang Ni;Xinyang Bai;Ruize Lin;K. Xiao
核因子 Y (NF-Y) 转录因子成员在调节与非生物胁迫反应相关的生理过程中发挥着重要作用。在这项研究中，我们表征了TaNF-YA7-5B（一种编码小麦NY-YA亚基的基因）在介导植物适应PEG诱导的脱水胁迫中的作用。TaNF-YA7-5B与其在不同植物物种中的同源物具有高度相似性。 TaNF-YA7-5B 蛋白通过其保守结构域被指定为植物 NF-YA 成员，并在内质网分类后靶向细胞核。酵母双杂交实验表明TaNF-YA7-5B与NF-YB和NF-YC亚家族的两个成员TaNF-YB2和TaNF-YC7相互作用，表明TaNF-YA7-5B和以上NF-YB和-YC伙伴构成异源三聚体。TaNF-YA7-5B在干旱时表现出诱导表达，其PEG诱导脱水转录本升高在正常恢复条件下恢复，表明其通过改变转录效率参与植物 PEG 诱导的脱水反应。过表达TaNF-YA7-5B使植物在PEG诱导的脱水下生长得到改善，这主要归因于调节气孔关闭和叶片保水、渗透剂生物合成和细胞ROS稳态的基因功能。 P5CS基因TaP5CS2和抗氧化酶（AE）基因TaSOD3、TaCAT1和TaPOD4的表达分别与TaNF-YA7-5B过表达和敲低相关，上调和下调；对它们的转基因分析验证了它们在 PEG 诱导脱水下正调节脯氨酸积累和 ROS 清除的功能。 RNA-seq 分析揭示了 TaNF-YA7-5Ben 的众多基因的转录被修饰，丰富了 GO 术语“生物过程”、“细胞成分”和“分子功能”。因此，TaNF-YA7-5B通过综合整合与干旱驯化相关的多种生理过程，成为植物适应干旱的重要调节因子。
Members of nuclear factor-Y (NF–Y) transcription factors play important roles in regulating physiological processes associated with abiotic stress responses. In this study, we characterizedTaNF-YA7-5B, a gene encoding wheat NY-YA subunit, in mediating plant adaptation to PEG-inducing dehydration stress.TaNF-YA7-5Bshares high similarities to its homologs across various plant species. The TaNF-YA7-5B protein is specified by its conserved domains as plant NF-YA members and targets onto nucleus after endoplasmic reticulum assortment. Yeast two-hybrid assays indicated that TaNF-YA7-5B interacts with TaNF-YB2 and TaNF-YC7, two members of NF-YB and NF-YC subfamilies, suggesting a heterotrimer constituted by TaNF-YA7-5B and above NF-YB and -YC partners.TaNF-YA7-5Bdisplayed induced expression upon drought and whose PEG-inducing dehydration-elevated transcripts were restored under normal recovery condition, suggesting its involvement in plant PEG-inducing dehydration response through modifying transcription efficiency. OverexpressingTaNF-YA7-5Bconferred plant improved growth under PEG-inducing dehydration, which was ascribed largely to the gene function in regulating stomata closing and leaf water retention, osmolyte biosynthesis, and cellular ROS homeostasis. The expression of P5CS geneTaP5CS2and antioxidant enzyme (AE) genes, namely,TaSOD3,TaCAT1, andTaPOD4, was upregulated and downregulated in lines with overexpression and knockdown ofTaNF-YA7-5B, respectively; transgene analysis on them validated their functions in positively regulating proline accumulation and ROS scavenging under PEG-inducing dehydration. RNA-seq analysis revealed modified transcription of numerous genes underlyingTaNF-YA7-5Benriched by GO terms ‘biological process’, ‘cellular components’, and ‘molecular function’. Therefore,TaNF-YA7-5Bis a crucial regulator for plant drought adaptation through comprehensively integrating diverse physiological processes associated with drought acclimation.