Distinct spatiotemporal expression of EFA6D, a guanine nucleotide exchange factor for ARF6, among the EFA6 family in mouse brain
Distinct spatiotemporal expression of EFA6D, a guanine nucleotide exchange factor for ARF6, among the EFA6 family in mouse brain
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DOI：
10.1016/j.brainres.2006.02.058
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发表时间：
2006-06
期刊：
影响因子：
2.9
通讯作者：
H. Sakagami;Hiroharu Suzuki;Akifumi Kamata;Y. Owada;K. Fukunaga;H. Mayanagi;H. Kondo
中科院分区：
文献类型：
--
作者：
H. Sakagami;Hiroharu Suzuki;Akifumi Kamata;Y. Owada;K. Fukunaga;H. Mayanagi;H. Kondo
EFA6家族是鸟嘌呤核苷酸交换因子（gef）的成员，可以在体外特异性激活ARF6。在这项研究中，我们确定了小鼠EFA6D编码一个1004个氨基酸的蛋白的完整初级序列，计算分子量为111,207 Da。在ARF下拉实验中，EFA6D对ARF6表现出优先的GEF活性。RT-PCR分析显示EFA6D在组织中分布广泛，脑组织中EFA6A、C、D高表达。原位杂交分析显示EFA6D与EFA6A、C在小鼠脑中的时空表达模式明显不同。此外，免疫印迹分析显示，EFA6D在突触后密度部分高度集中。这些发现表明，大脑中EFA6家族的三个成员对ARF6的时空调节存在差异。
The EFA6 family is a member of guanine nucleotide exchange factors (GEFs) that can activate ARF6 specifically in vitro. In this study, we determined the complete primary sequence of mouse EFA6D encoding a protein of 1004 amino acids with a calculated molecular weight of 111,207 Da. In ARF pull-down assay, EFA6D showed a preferential GEF activity toward ARF6. RT-PCR analysis revealed the widespread tissue distribution of EFA6D and the high expression of EFA6A, C and D in the brain. In situ hybridization analysis demonstrated a distinct spatiotemporal expression pattern of EFA6D from those of EFA6A and C in mouse brain. Furthermore, immunoblot analysis revealed that EFA6D was highly concentrated in the postsynaptic density fraction. These findings suggest differential spatiotemporal regulation of ARF6 by three members of the EFA6 family in the brain.