Characterization of the BspA and Pmp protein family of trichomonads
Characterization of the BspA and Pmp protein family of trichomonads
复制标题
DOI：
10.1186/s13071-019-3660-z
复制
发表时间：
2019-08-19
影响因子：
3.2
通讯作者：
Gould, Sven B.
中科院分区：
文献类型：
--
作者：
Handrich, Maria R.;Garg, Sriram G.;Gould, Sven B.
背景阴道毛滴虫是一种感染人类的滴虫，考虑到其寄生生活方式，它是目前研究得最好的一种，也是唯一一种有完整基因组序列的滴虫。迷走神经编码异常多的蛋白质。许多基因家族被大量扩增，并且推测一些基因已经从原核来源获得。在后者中，有两个基因家族，其具有与拟杆菌目/螺旋体目和衣原体目的蛋白质共享相似性的结构域：分别为BspA和Pmp蛋白质。结果我们对5种毛滴虫的转录组进行了测序，筛选出了含有BspA和Pmp结构域的蛋白，并对毛滴虫中两个家族的候选蛋白进行了表征。流浪汉在这里，我们证明（i）BspA和Pmp结构域的蛋白质是通用的毛滴虫，但在T。迷走神经;（ii）与内吞机制的同时扩增一致，存在大量携带C-末端内吞基序的BspA和Pmp蛋白;和（iii）两个家族通过ER运输并且具有增加非毒性T中的粘附性能的能力。迷走神经炎菌株和鸡四毛滴虫通过迄今未知的机制。结论本研究初步揭示了这两个广泛分布的蛋白家族的功能特征，有助于更好地理解毛滴虫BspA和Pmp结构域的起源和进化。
Background Trichomonas vaginalis is a human-infecting trichomonad and as such the best studied and the only for which the full genome sequence is available considering its parasitic lifestyle, T. vaginalis encodes an unusually high number of proteins. Many gene families are massively expanded and some genes are speculated to have been acquired from prokaryotic sources. Among the latter are two gene families that harbour domains which share similarity with proteins of Bacteroidales/Spirochaetales and Chlamydiales: the BspA and the Pmp proteins, respectively. Results We sequenced the transcriptomes of five trichomonad species and screened for the presence of BspA and Pmp domain-containing proteins and characterized individual candidate proteins from both families in T. vaginalis. Here, we demonstrate that (i) BspA and Pmp domain-containing proteins are universal to trichomonads, but specifically expanded in T. vaginalis; (ii) in line with a concurrent expansion of the endocytic machinery, there is a high number of BspA and Pmp proteins which carry C-terminal endocytic motifs; and (iii) both families traffic through the ER and have the ability to increase adhesion performance in a non-virulent T. vaginalis strain and Tetratrichomonas gallinarum by a so far unknown mechanism. Conclusions Our results initiate the functional characterization of these two broadly distributed protein families and help to better understand the origin and evolution of BspA and Pmp domains in trichomonads.