Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier
Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier
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DOI：
10.1007/s10529-007-9463-x
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发表时间：
2007-11-01
影响因子：
2.7
通讯作者：
Ahn, Taeho
中科院分区：
文献类型：
--
作者：
Kim, Mihee;Yun, Chul-Ho;Ahn, Taeho
我们描述了一种以聚合物珠为载体制备抗肽抗原(细胞色素P450 1A2和3A4的表位区、HA标签、FLAG标签和c-myc标签)的多克隆抗体(Pab)的方法。用化学交联剂将直径分别为0.05、0.5、1和2微米的羧化聚苯乙烯小球与被称为表位区域的多肽偶联并注射到兔体内。通过每隔两周皮内免疫动物两次，用抗血清进行免疫印迹试验表明有效地产生了多克隆抗体。
We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 mu m diam) were conjugated with the peptide, which is known as an epitope region, using a chemical cross-linker and injected into rabbits with adjuvant. By immunizing the animals intradermally twice at two week intervals, an immunoblot assay with anti-sera showed the efficient generation of the PAb.