The direct isolation of the mitotic apparatus.
The direct isolation of the mitotic apparatus.
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DOI：
10.1083/jcb.10.4.467
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发表时间：
1961-08
期刊：
影响因子：
--
通讯作者：
HARRIS P
中科院分区：
文献类型：
--
作者：
MAZIA D;MITCHISON JM;MEDINA H;HARRIS P
描述了一种不使用乙醇或洗涤剂从分裂海胆卵中分离有丝分裂器的方法。在本方法中，将卵直接分散在含有1 M（至1.15 M）蔗糖、0.15 M二硫二甘醇和0.001 M茴香的培养基中，pH为6，释放出明显完整的有丝分裂器。该方法适用于酶活性、脂质成分和影响仪器稳定性的变量的研究。
A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.