The dosage of small volumes for chromatographic quantifications using a drop-on-demand dispenser system
The dosage of small volumes for chromatographic quantifications using a drop-on-demand dispenser system
复制标题
DOI：
10.1007/s00216-007-1335-7
复制
发表时间：
2007-07-01
影响因子：
4.3
通讯作者：
Schmitt-Kopplin, Philippe
中科院分区：
文献类型：
--
作者：
Englmann, Matthias;Fekete, Agnes;Schmitt-Kopplin, Philippe
在对高丝氨酸进行定量超高效液相色谱 (UPLC) 分析之前，测试了市售的压电驱动按需滴液分配器是否适用于制备分析校准标准品以及标准添加方法。测试了按需滴加系统的重现性，并通过从 1,000.0 mg/L 储备溶液制备一系列 1.0 mg/L 咖啡因标准溶液并通过 UPLC 分析获得的浓度来验证液滴体积。测量之间的重复性优于 1% 相对标准偏差，每天最高可达 1.6%。将结果与生成标准溶液的传统方法（移液）进行比较。对测定平均单滴体积的重量法和基于摄影的方法进行了比较，发现两者非常一致。该系统用于通过在含有该分析物的细菌培养物上清液中添加标准品来定量 N-癸酰高丝氨酸。与传统定量技术的一致性很高。本文展示了该方法的可行性，具有样品和溶剂体积消耗低、重现性和可靠性非常好的优点以及易于使用的优点。
A commercially available piezo-driven drop-on-demand dispenser was tested for its suitability for the preparation of analytical calibration standards and in a standard addition approach prior to quantitative ultra performance liquid chromatography (UPLC) analysis of homoserines. The reproducibility of the drop-on-demand dosing system was tested and the verification of the droplet volume was performed by preparing a series of 1.0 mg/L caffeine standard solutions from a 1,000.0 mg/L stock solution and analysis of the concentrations obtained by UPLC. The reproducibility was better than 1% relative standard deviation from measurement to measurement and the highest was 1.6% from day to day. The results were compared with the conventional way of generating standard solutions (pipetting). A gravimetric method and a photography-based method for the determination of the average single droplet volume were compared and found to be in very good agreement. The system was employed for the quantification of N-decanoyl homoserine by standard addition in bacterial culture supernatants containing this analyte. The agreement with conventional quantification techniques was high. The paper shows the feasibility of the approach with advantages in low sample and solvent volume consumption and very good reproducibility and reliability combined with easy usage.